B6;129S2-Sell^tm2Hyn/J

Stock number: 002917
Product name: L-selectin KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Detailed description

Mice homozygous for the Sell^tm2Hyn mutation are viable and fertile. They are characterized by hypoplastic lymph nodes because of failure of lymphocytes to roll on high endothelial venules in the nodes.

Development

A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells and maintained on a mixed C57BL/6 and 129S2 background.

Allele

Symbol: Sell^tm2Hyn
Name: targeted mutation 2, Richard Hynes
MGI accession ID: MGI:3839975
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sell
Marker name: selectin, lymphocyte
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. ES cells heterozygote for the Selp^tm1Hyn and Sele^tm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of the L-selectin protein but not the two other selectin proteins in leukocytes isolated from homozygotes. Thus, this targeted mutation occurred in trans with the other two null mutations and were subsequently bred apart.