Mice homozygous for the Selltm2Hyn mutation are viable and fertile. They are characterized by hypoplastic lymph nodes because of failure of lymphocytes to roll on high endothelial venules in the nodes.
Dr. Richard Hynes, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sell | selectin, lymphocyte |
Mice homozygous for the Selltm2Hyn mutation are viable and fertile. They are characterized by hypoplastic lymph nodes because of failure of lymphocytes to roll on high endothelial venules in the nodes.
A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells and maintained on a mixed C57BL/6 and 129S2 background.
Allele Name | targeted mutation 2, Richard Hynes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | L-selectin- |
Gene Symbol and Name | Sell, selectin, lymphocyte |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. ES cells heterozygote for the Selptm1Hyn and Seletm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of the L-selectin protein but not the two other selectin proteins in leukocytes isolated from homozygotes. Thus, this targeted mutation occurred in trans with the other two null mutations and were subsequently bred apart. |
The donating lab maintained these mice by avoiding brother-sister matings. Expected coat color from breeding:Black
When using the L-selectin KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002917 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sell<tm1Hyn> |
Frozen Mouse Embryo | B6;129S2-Sell<tm2Hyn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Sell<tm2Hyn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Sell<tm2Hyn>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Sell<tm2Hyn>/J Frozen Embryos | $3373.50 |
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