These Gria2 knock-out mice exhibit an increase in Ca2+ permeability and enhanced long-term potentiation in neurons. They are suitable for use in applications related to the study of Ca2+ permeability and plasticity of neurons in the central nervous system.
Dr. John Roder, University of Toronto
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gria2 | glutamate receptor, ionotropic, AMPA2 (alpha 2) |
Mice homozygous for the Gria2tm1Rod targeted mutation (GluR2) were originally reported to be about one half the size of their normal littermates by two to three weeks leading to some preweaning mortality. Growth retardation and mortality can be diminished by reducing litter size and eliminating some competition for the mother's milk. Homozygotes do appear similar in size and weight by eight weeks of age. Neurons lacking Gria2 exhibit a 9-fold increase in Ca2+ permeability and enhanced long-term potentiation (LTP). Antagonists of AMPA and NMDA receptors block all the enhanced LTP seen in homozygous null mice. Unpublished data show changes in pain thresholds, sensitivity to anaesthetics, focal cerebral ischemia, apoptosis of neuronal subpopulations and learning impairments.
It has been the experience of The Jackson Laboratory that no homozygous animals can be recovered from heterozygous sibling matings.
Allele Name | targeted mutation 1, John Roder |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GluA2; GluR2- |
Gene Symbol and Name | Gria2, glutamate receptor, ionotropic, AMPA2 (alpha 2) |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 3 |
Molecular Note | A neomycin resistance cassette replaced exon 11 of the gene, which encodes the pore loop and transmembrane region, TM1. Western blots of brain plasma membranes from homozygous mutant mice showed no detectable protein for the targeted gene. |
Mutations Made By | Dr. John Roder, University of Toronto |
When maintaining a live colony, heterozygous mice may be bred with wildtype siblings. In our colonies at The Jackson Laboratory, no homozygous offspring are produced from heterozygous breedings. The expected coat color from breeding is Albino,Gray.
When using the GluR2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002913 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gria2<tm1Rod>/ |
Frozen Mouse Embryo | STOCK Gria2<tm1Rod>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gria2<tm1Rod>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gria2<tm1Rod>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Gria2<tm1Rod>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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