STOCK Gria2^tm1Rod/J

Stock number: 002913
Product name: GluR2 KO
Research areas: Cell Biology Research, Neurobiology Research

Description

These Gria2 knock-out mice exhibit an increase in Ca²⁺ permeability and enhanced long-term potentiation in neurons. They are suitable for use in applications related to the study of Ca²⁺ permeability and plasticity of neurons in the central nervous system.

Detailed description

Mice homozygous for the Gria2^tm1Rod targeted mutation (GluR2) were originally reported to be about one half the size of their normal littermates by two to three weeks leading to some preweaning mortality. Growth retardation and mortality can be diminished by reducing litter size and eliminating some competition for the mother's milk. Homozygotes do appear similar in size and weight by eight weeks of age. Neurons lacking Gria2 exhibit a 9-fold increase in Ca2+ permeability and enhanced long-term potentiation (LTP). Antagonists of AMPA and NMDA receptors block all the enhanced LTP seen in homozygous null mice. Unpublished data show changes in pain thresholds, sensitivity to anaesthetics, focal cerebral ischemia, apoptosis of neuronal subpopulations and learning impairments.

It has been the experience of The Jackson Laboratory that no homozygous animals can be recovered from heterozygous sibling matings.

Allele

Symbol: Gria2^tm1Rod
Name: targeted mutation 1, John Roder
MGI accession ID: MGI:1857436
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gria2
Marker name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Chromosome: 3
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin resistance cassette replaced exon 11 of the gene, which encodes the pore loop and transmembrane region, TM1. Western blots of brain plasma membranes from homozygous mutant mice showed no detectable protein for the targeted gene.