Mice homozygous for the Trp53tm1Tyj mutation show no visible phenotype but most develop tumors (principally lymphomas and sarcomas) at 3-6 months of age. Heterozygous mice develop tumors at about 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome, a form of familial breast cancer with mutations in TRP53. Homozygous mice may produce a litter before succumbing to tumors.
The Trp53tm1Tyj mutant strain was developed in the laboratory of Dr. Tyler Jacks at the Center for Cancer Research at the Massachusetts Institute of Technology. The 129-derived D3 ES cell line was used. The FVB/J strain was produced by backcrossing the Trp53tm1Tyj mutation 5 times to FVB/J mice.
|Allele Name||targeted mutation 1, Tyler Jacks|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Trp53-; Trp53KO; p53-; p53delta; p53null; p53KO|
|Gene Symbol and Name||Trp53, transformation related protein 53|
|Gene Synonym(s)||BCC7; LFS1; P53; TRP53; p44; p53|
|Site of Expression||Normal Trp53 expression is widespread.|
|Strain of Origin||129S2/SvPas|
|General Note||This mutant allele was produced by a targeted neo insertion into the Trp53 locus. Homozygotes show no visible phenotype but develop tumors at 3-6 months of age. Heterozygotes develop tumors at 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome (OMIM 151623), a form of familial breast cancer with mutations in TRP53 (J:16022)(J:16023) A specific human mutation found in hepatocellular carcinomas caused by hepatitis B infection or by aflatoxin exposure has been created in a mouse model, resulting in a similar gene product (J:27363).|
|Molecular Note||A neomycin cassette replaced 40% of the coding sequences beginning with exon 2 (upstream of the translation start site) and extending into exon 6.|
|Mutations Made By|| |
Dr. Tyler Jacks, Massachusetts Institute of Technology
Homozygotes show no visible phenotype but most develop tumors at 3-6 months of age (principally lymphomas and sarcomas). Heterozygous mice develop tumors at about 10 months of age. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to FVB/NJ inbred mice (Stock No. 001800). The expected coat color from breeding is albino.
When using the p53Δ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002899 in your Materials and Methods section.
|Hemizygous or Non Carrier for Trp53<tm1Tyj>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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