Wnt-4 is expressed in kidney mesenchyme and its derivatives. It encodes a secreted glycoprotein that is required for kidney tubulogenesis. Mice lacking Wnt-4 activity fail to form pretubular cell aggregates; however, other aspects of mesenchymal and ureteric development are unaffected. Mice carrying this knock-out die around 24 hours after birth, most likely due to failure in kidney function.
A targeting vector containing a PGK-neo cassette was used to disrupt exon 3. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ mice.
|Allele Name||targeted mutation 1, Andrew P McMahon|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Wnt-4-; Wnt4n|
|Gene Symbol and Name||Wnt4, wingless-type MMTV integration site family, member 4|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exon 3, encoding amino acids 106 through 196, was replaced by a PGK-neo cassette. The occasional expression of the targeted locus was observed in poorly developed aggregates in the kidneys of homozygous mutant mice via in situ hybridization. Expression was unaltered in the central nervous system and mesonephric mesenchyme.|
|Mutations Made By|| |
Andrew McMahon, University of Southern California
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448). Mice homozygous for the mutation die 24 hours after birth.
When using the Wnt-4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002866 in your Materials and Methods section.