B6;129S4-Jak3^tm1Ljb/J

Stock number: 002852
Product name: Jak-3-
Research areas: Immunology, Inflammation and Autoimmunity Research, Mouse/Human Gene Homologs, Research Tools

Description

Mice homozygous for Jak3^tm1Ljb have blocked B cell development at pre-B resulting in a significant decrease in peripheral IgM⁺ B cells. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.

Detailed description

Mice homozygous for the Jak3^tm1Ljb mutation are viable and fertile. B cell development is blocked at pre-B resulting in a significant decrease in peripheral IgM⁺ B cells. The thymus is small but T cell development is relatively normal. There are increased numbers of CD4⁺ Cd8^- cells in some homozygotes. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.

Development

Subdomains I to IV of the kinase domain region of the Jak3 gene were replaced using a vector containing the neo resistance gene. The Donating Investigator reports that the chimeric animals were backcrossed to C57BL/6 for one generation.

Allele

Symbol: Jak3^tm1Ljb
Name: targeted mutation 1, Leslie J Berg
MGI accession ID: MGI:1857276
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Jak3
Marker name: Janus kinase 3
Chromosome: 8
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a 0.6 kb fragment of the gene, which contains sequences encoding subdomains I to IV of the kinase domain. Protein immunoblots of lysates from bone marrow, spleen, and thymus showed no targeted protein.