Mice homozygous for Jak3tm1Ljb have blocked B cell development at pre-B resulting in a significant decrease in peripheral IgM+ B cells. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.
Dr. Leslie Berg, University of Massachusetts Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Jak3 | Janus kinase 3 |
Mice homozygous for the Jak3tm1Ljb mutation are viable and fertile. B cell development is blocked at pre-B resulting in a significant decrease in peripheral IgM+ B cells. The thymus is small but T cell development is relatively normal. There are increased numbers of CD4+ Cd8- cells in some homozygotes. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.
Allele Name | targeted mutation 1, Leslie J Berg |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Jak-3-; Jak3- |
Gene Symbol and Name | Jak3, Janus kinase 3 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 8 |
Molecular Note | A neomycin resistance cassette replaced a 0.6 kb fragment of the gene, which contains sequences encoding subdomains I to IV of the kinase domain. Protein immunoblots of lysates from bone marrow, spleen, and thymus showed no targeted protein. |
Mutations Made By | Dr. Leslie Berg, University of Massachusetts Medical School |
Colony is currently maintained homozygote x homozygote. Expected coat color from breeding is black or agouti.
When using the B6;129S4-Jak3tm1Ljb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002852 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Jak3<tm1Ljb> |
Frozen Mouse Embryo | B6;129S4-Jak3<tm1Ljb>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Jak3<tm1Ljb>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Jak3<tm1Ljb>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Jak3<tm1Ljb>/J Frozen Embryos | $3373.50 |
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