B6;129S4-Slc4a1^tm1Llp/J

Stock number: 002832
Product name: AE1-
Research areas: Hematological Research, Neurobiology Research

Description

These Slc4a1 knock-out mice exhibit severe hemolytic anemia.

Detailed description

These mice show severe hemolytic anemia with 85% homozygotes dying before weaning. Surviving homozygous adult Slc4a1^tm1Llp mice are smaller than their normal littermates and spleens are more than 15 times the size of normal littermate spleens. Red blood cells from homozygous mice show severe spherocytosis and hemolysis, but have an intact membrane skeleton.

Allele

Symbol: Slc4a1^tm1Llp
Name: targeted mutation 1, Luanne L Peters
MGI accession ID: MGI:1857140
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Slc4a1
Marker name: solute carrier family 4 (anion exchanger), member 1
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: Sequence from the within the 3' region of exon 9 to the middle of exon 11 was replaced with a neomycin selection cassette. An absence of transcript was determined by Northern blot analysis of reticulocyte RNA derived from newborns and fetal liver RNA, both obtained from homozygous mutant mice. Western analysis and immunohistochemical analysis showed an absence of the encoded protein in homozygous mutant animals.
General note: In homozygotes for this and other targeted null mutations of the AE1 gene, severe spherocytosis and hemolysis (J:35487, J:35637) result from loss of erythrocyte membrane components, but the membrane skeleton is intact. Glycophorin A, the major transmembrane sialoglycoprotein of the erythrocyte, contributes to the expression of blood group antigens, and serves other erythrocyte membrane functions. It is never incorporated into red blood cell membranes of null mutant homozygotes (J:46738).