Mice homozygous for the E2f1 knock-out show thymocyte maturation defects due to a failure of apoptosis, eventually resulting in increased proliferation and increased tumorigenesis. These mice may be useful in studies of apoptosis, cancer, thymocyte development/selection, diabetes, autoimmunity, and multiple sclerosis.
Dr. Michael E. Greenberg, Harvard Medical School
Mice homozygous for defective E2f1 are viable and fertile. They show thymocyte maturation defects due to a failure of apoptosis, eventually resulting in increased proliferation and increased tumorigenesis. As mutant mice age, they show exocrine gland dysplasia and testicular atrophy. Mutant mice develop a broad spectrum of cancers, although mammary carcinomas were not observed on this genetic background. Mutant mice are also protected from experimental autoimmune encephalomyelitis (EAE). These mice may be useful in studies of apoptosis, cancer, thymocyte development/selection, diabetes, autoimmunity, and multiple sclerosis.
This strain was developed by Dr. Seth Field in the laboratory of Dr. Michael Greenberg at Children's Hospital, Boston, MA. The DNA binding domain and part of the dimerization domain of the E2f1 gene were interrupted by a vector containing the neomycin resistance gene.
|Allele Name||targeted mutation 1, Michael E Greenburg|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||E2f1-; E2F-1-|
|Gene Symbol and Name||E2f1, E2F transcription factor 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Part of exon 3, intron 3 and exon 4 were replaced with a neomycin cassette. These sequences encode residues encoding the leucine zipper and those critical for DNA binding. Northern blot analysis did not detect a transcript made from this allele in fibroblasts isolated from homozygous embryos.|
|Mutations Made By|| |
Dr. Seth Field, Harvard Medical School
This strain is currently on a mixed C57BL/6 x 129 genetic backgrounds. Litter sizes are reported to be 5-6 pups for these mixed background mice. Homozygotes viable and fertile. Expected coat color from breeding:White Bellied Agouti
When using the E2F-1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002785 in your Materials and Methods section.