B6.129S4-Cdkn1b^tm1Mlf/J

Stock number: 002781
Product name: p27-
Research areas: Cancer Research, Cell Biology Research, Reproductive Biology Research

Description

Homozygous mice of the cyclin-dependent kinase inhibitor 1B (Cdkn1b) knock-out (also referred to as p27^kip) exhibit multi-organ hyperplasia and develop pituitary tumors. These mice may be useful in studies of tumorigenesis.

Detailed description

Mice deficient in p27^kip are viable and larger than normal littermates, with increased cellularity of all tissues. The thymus and spleen are particularly enlarged. Homozygous (nullizygous) adult mice have a shortened lifespan due to the growth of benign intermediate lobe pituitary tumors. Female mice are infertile, with a follicular phase ovulatory block. Large doses of exogenous gonadotropin induce ovulation, but both implantation and intrauterine embryonic development is impaired. The mice demonstrate haploid-insufficient susceptibility to the development of adenomas in the pituitary, intestine and lung adenomas following exposure to gamma irradiation or chemical carcinogens.

Development

This strain was generated by Dr. Matthew Fero at the Fred Hutchinson Cancer Research Center. The targeting construct contains a neo resistance gene and replaces the entire coding region of the Cdkn1b gene. 129S4/SvJaeSor-derived AK7 ES cells were used and the mutation was subsequently backcrossed to C57BL/6 mice.

Allele

Symbol: Cdkn1b^tm1Mlf
Name: targeted mutation 1, Matthew Fero
MGI accession ID: MGI:1857146
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cdkn1b
Marker name: cyclin-dependent kinase inhibitor 1B
Chromosome: 6
Strain of origin: 129S4/SvJaeSor
Molecular note: Replacement of the entire coding region of the Cdkn1b gene with a neomycin cassette.