These Nfe2 knock-out mice exhibit 90% embryonic mortality with thrombocytopenia. They are suitable for use in applications related to the study of megakaryocyte maturation and platelet production.
IMR Colony, The Jackson Laboratory
90% of the homozygotes die within the first week of life. They display profound thrombocytopenia (no detectable circulating blood platelets) and megakaryocytes that show a maturation arrest characterized by failure to organize demarcatoin membranes and delimit platelet territories. Those that survive are anemic and display additional sporadic mortality of 10-20%. Male homozygotes are fertile.
|Allele Name||targeted mutation 1, Stuart Orkin|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||NF-E2-; p45 NF-E2-; p45NFE2-|
|Gene Symbol and Name||Nfe2, nuclear factor, erythroid derived 2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A PGK-neomycin cassette was inserted into exon 3, immediately upstream of sequences required for DNA binding and protein dimerization. Absence of intact mRNA and protein were demonstrated by Northern and immunoblot analyses, respectively.|
|Mutations Made By|| |
Stuart Orkin, Harvard University