Type I interferon gene induction is abnormal in homozygous mice. They exhibit impairment of CD8+ T cell and NK cell maturation, impaired IL-12 macrophage production, exclusive Th2 differentiation, and defective Th1.
Dr. Ronald Schwartz, National Institutes of Health
Genetic Background | Generation |
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N11F50
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Irf1 | interferon regulatory factor 1 |
Mice homozygous for the Irf1tm1Mak targeted mutation are viable and fertile with no major abnormalities. Type I interferon gene induction is abnormal. These mice exhibit impairment of CD8+ T cell and NK cell maturation, impaired IL-12 macrophage production, exclusive Th2 differentiation, and defective Th1. While these mice are highly susceptible to infections, they have been reported to be resistant to several autoimmune diseases such as collagen-induced arthritis, experimental autoimmune encephalomyelitis, Helicobacter pylori-induced gastritis, induced lymphocytic thyroiditis, insulitis, or diabetes.
A neomycin resistance cassette replaced a 1.2 kb region of the gene, which encodes for amino acids 63 - 223, deleting part of the DNA-binding domain of the protein. The construct was electroporated into D3 ES cells and correctly targeted cells were injected into C57BL/6J blastocysts.
Allele Name | targeted mutation 1, Tak Mak |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IRF-1- |
Gene Symbol and Name | Irf1, interferon regulatory factor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced a 1.2 kb region of the gene, which encodes for amino acids 63 - 223, deleting part of the DNA-binding domain of the protein. |
Mutations Made By | Dr. Tak Mak, University Health Network/Un of Toronto |
When maintaining the live colony, homozygous mice may be bred together. The expected coat color from breeding is Black.
When using the Irf1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002762 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Irf1<tm1Mak> |
Frozen Mouse Embryo | B6.129S2-Irf1<tm1Mak>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Irf1<tm1Mak>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Irf1<tm1Mak>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Irf1<tm1Mak>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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