B6.129S2-Irf1^tm1Mak/J

Stock number: 002762
Product name: Irf1 KO
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Type I interferon gene induction is abnormal in homozygous mice. They exhibit impairment of CD8⁺ T cell and NK cell maturation, impaired IL-12 macrophage production, exclusive Th2 differentiation, and defective Th1.

Detailed description

Mice homozygous for the Irf1^tm1Mak targeted mutation are viable and fertile with no major abnormalities. Type I interferon gene induction is abnormal. These mice exhibit impairment of CD8⁺ T cell and NK cell maturation, impaired IL-12 macrophage production, exclusive Th2 differentiation, and defective Th1. While these mice are highly susceptible to infections, they have been reported to be resistant to several autoimmune diseases such as collagen-induced arthritis, experimental autoimmune encephalomyelitis, Helicobacter pylori-induced gastritis, induced lymphocytic thyroiditis, insulitis, or diabetes.

Development

A neomycin resistance cassette replaced a 1.2 kb region of the gene, which encodes for amino acids 63 - 223, deleting part of the DNA-binding domain of the protein. The construct was electroporated into D3 ES cells and correctly targeted cells were injected into C57BL/6J blastocysts.

Allele

Symbol: Irf1^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1857205
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: IRF-1^-
Marker symbol: Irf1
Marker name: interferon regulatory factor 1
Marker synonyms: Irf-1; MAR
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette replaced a 1.2 kb region of the gene, which encodes for amino acids 63 - 223, deleting part of the DNA-binding domain of the protein.