| Allele Name | transgene insertion 53, Stephen M Hedrick |
| Allele Type | Transgenic (Inserted expressed sequence) |
| Allele Synonym(s) | AND; AND TCR; CC; TcrAND; Tg(TcrAND) |
| Gene Symbol and Name | Tg(TcrAND)53Hed, transgene insertion 53, Stephen M Hedrick |
| Gene Synonym(s) | |
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
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| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
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| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
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| Strain of Origin | (C57BL/6 x SJL)F2 |
| Chromosome | UN |
| General Note | The transgenic TCR is expressed on virtually all T cells in mice expressing Ek. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragment of pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4+CD8- cells, a peripheral T-cell population is almost exclusively CD4+ and show no expression of I-Ek. |
| Molecular Note | Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the Ek molecule. The alpha-chain of this transgenic TCR differs by one amino acid (an alanine instead of a threonine at position 99) to the alpha chain of the transgenic TCR expressed in Tg(TcraTcrbAD10)1Hed transgenic mice. |
| Mutations Made By | Dr. Stephen Hedrick, University of California at San Diego |
