B10.Cg-Tg(TcrAND)53Hed/J

Stock number: 002761
Product name: αβ TCR
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These αβ TCR mice express a transgene containing rearranged TCR alpha and beta genes with endogenous promoter and enhancer sequences.

Detailed description

Mice carrying the (TcrAND)53Hed transgene express a rearranged T-cell receptor (V alpha 11.1 / V beta 3) specific for the carboxy-terminal fragment of pigeon cytochrome c and the E^k molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. There are an abnormally high percentage of mature CD4⁺CD8^- cells. The peripheral T-cell population is almost exclusively CD4⁺. The original C57BL/6 and SJL mixed background strain (Stock number 002408) was backcrossed to C57BL/10 to create this strain. Both strains are fixed for H2^b. Because C57BL/10 mice do not express I-E, this mouse must be crossed to a strain that expresses I-E^k to study the interaction of the transgenic T-cell receptor with the pigeon cytochrome c antigen. The lack of I-E^k expression in the transgenic line allows it to serve as a universal donor for crossing the transgene onto other strains expressing I-E^k.

Allele

Symbol: Tg(TcrAND)53Hed
Name: transgene insertion 53, Stephen M Hedrick
MGI accession ID: MGI:3053044
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcrAND)53Hed
Marker name: transgene insertion 53, Stephen M Hedrick
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: Tcrb,T cell receptor beta chain,mouse, laboratory; Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the E^k molecule. The alpha-chain of this transgenic TCR differs by one amino acid (an alanine instead of a threonine at position 99) to the alpha chain of the transgenic TCR expressed in Tg(TcraTcrbAD10)1Hed transgenic mice.
General note: The transgenic TCR is expressed on virtually all T cells in mice expressing E^k. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragment of pigeon cytochrome c and the E^k molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4⁺CD8^- cells, a peripheral T-cell population is almost exclusively CD4⁺ and show no expression of I-E^k.