These αβ TCR mice express a transgene containing rearranged TCR alpha and beta genes with endogenous promoter and enhancer sequences.
Dr. Ronald Schwartz, National Institutes of Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice carrying the (TcrAND)53Hed transgene express a rearranged T-cell receptor (V alpha 11.1 / V beta 3) specific for the carboxy-terminal fragment of pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. There are an abnormally high percentage of mature CD4+CD8- cells. The peripheral T-cell population is almost exclusively CD4+. The original C57BL/6 and SJL mixed background strain (Stock number 002408) was backcrossed to C57BL/10 to create this strain. Both strains are fixed for H2b. Because C57BL/10 mice do not express I-E, this mouse must be crossed to a strain that expresses I-Ek to study the interaction of the transgenic T-cell receptor with the pigeon cytochrome c antigen. The lack of I-Ek expression in the transgenic line allows it to serve as a universal donor for crossing the transgene onto other strains expressing I-Ek.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 53, Stephen M Hedrick |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | AND; AND TCR; CC; TcrAND; Tg(TcrAND) |
Gene Symbol and Name | Tg(TcrAND)53Hed, transgene insertion 53, Stephen M Hedrick |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | The transgenic TCR is expressed on virtually all T cells in mice expressing Ek. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragment of pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4+CD8- cells, a peripheral T-cell population is almost exclusively CD4+ and show no expression of I-Ek. |
Molecular Note | Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the Ek molecule. The alpha-chain of this transgenic TCR differs by one amino acid (an alanine instead of a threonine at position 99) to the alpha chain of the transgenic TCR expressed in Tg(TcraTcrbAD10)1Hed transgenic mice. |
Mutations Made By | Dr. Stephen Hedrick, University of California at San Diego |
When maintaining a live colony, homozygous mice may be bred together. The expected coat color from breeding is black.
When using the αβ TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #002761 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(TcrAND)53Hed/ |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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