B6.Cg-Tg(hAGT)2041Sig/J

Stock number: 002749
Product name: HuAoGen
Research areas: Cardiovascular Research

Description

These HuAoGen mice express the human angiotensinogen transgene and may be useful when studying blood pressure and renal perfusion pressure.

Detailed description

This strain carries a human angiotensinogen transgene whose expression results in angiotensinogen plasma concentrations 150X that normally found in human plasma. These mice are normotensive but become transiently hypertensive when injected with a bolus of purified renin. A chronically hypertensive mouse can by created by breeding this strain with a strain carrying the human renin transgene (C57BL/6-TgN(hREN)9Sig; 002750). The mean arterial pressure of the double transgenic is ~155 mmHg (controls ~111 mmHg). Both systolic and diastolic blood pressures are elevated.

Development

A human genomic clone that included all five exons and intervening intron sequences was injected into B6SJLF2 eggs. The founder number for this strain is 204.1. In the nomenclature it is recorded as 2041 as punctuation marks are not allowed. Mice have been backcrossed to C57BL/6 for at least 7 generations.

Allele

Symbol: Tg(hAGT)2041Sig
Name: transgene insertion 2041, Curt Sigmund
MGI accession ID: MGI:2447399
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(hAGT)2041Sig
Marker name: transgene insertion 2041, Curt Sigmund
Chromosome: UN
Strain of origin: (C57BL/6 x SJL/J)F2
Expressed genes: AGT,angiotensinogen,human
Molecular note: The 14 kb transgenic construct contained the entire human angiotensinogen gene (approximately 11.5 kb) and 5' (1.2 kb) and 3' (1.4 kb) flanking sequence. Sequence analysis showed that the construct contained the M235T variant but not the T174M variant. The human AGT promoter drove high levels of expression in the liver and kidney. Expression was also detected in the heart, adrenal gland, and adipose tissue, and at very low levels in the brain and submandibular gland. Sexually dimorphic expression, in which expression was higher in males, was observed in the kidney and adrenal gland. Lines 204.1 (2041) and 204.3 (2043) were created and transgenic mice on a C57BL/6 background show angiotensinogen plasma concentrations 150X that normally found in human plasma.