These HuAoGen mice express the human angiotensinogen transgene and may be useful when studying blood pressure and renal perfusion pressure.
Dr. Curt Sigmund, University of Iowa College of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
This strain carries a human angiotensinogen transgene whose expression results in angiotensinogen plasma concentrations 150X that normally found in human plasma. These mice are normotensive but become transiently hypertensive when injected with a bolus of purified renin. A chronically hypertensive mouse can by created by breeding this strain with a strain carrying the human renin transgene (C57BL/6-TgN(hREN)9Sig; 002750). The mean arterial pressure of the double transgenic is ~155 mmHg (controls ~111 mmHg). Both systolic and diastolic blood pressures are elevated.
A human genomic clone that included all five exons and intervening intron sequences was injected into B6SJLF2 eggs. The founder number for this strain is 204.1. In the nomenclature it is recorded as 2041 as punctuation marks are not allowed. Mice have been backcrossed to C57BL/6 for at least 7 generations.
Expressed Gene | AGT, angiotensinogen, human |
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Site of Expression |
Allele Name | transgene insertion 2041, Curt Sigmund |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | 204/1 line; A+; hAng; Hu-AGT-TG |
Gene Symbol and Name | Tg(hAGT)2041Sig, transgene insertion 2041, Curt Sigmund |
Gene Synonym(s) | |
Promoter | AGT, angiotensinogen, human |
Expressed Gene | AGT, angiotensinogen, human |
Strain of Origin | (C57BL/6 x SJL/J)F2 |
Chromosome | UN |
Molecular Note | The 14 kb transgenic construct contained the entire human angiotensinogen gene (approximately 11.5 kb) and 5' (1.2 kb) and 3' (1.4 kb) flanking sequence. Sequence analysis showed that the construct contained the M235T variant but not the T174M variant. The human AGT promoter drove high levels of expression in the liver and kidney. Expression was also detected in the heart, adrenal gland, and adipose tissue, and at very low levels in the brain and submandibular gland. Sexually dimorphic expression, in which expression was higher in males, was observed in the kidney and adrenal gland. Lines 204.1 (2041) and 204.3 (2043) were created and transgenic mice on a C57BL/6 background show angiotensinogen plasma concentrations 150X that normally found in human plasma. |
Mutations Made By | Dr. Curt Sigmund, University of Iowa College of Medicine |
When using the HuAoGen mouse strain in a publication, please cite the originating article(s) and include JAX stock #002749 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(hAGT)2041Sig |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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