CAn.129S(B6)-Cd74^tm1Liz/J

Stock number: 002730
Product name: CD74-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Cd74 (formerly Ii) knock-out mice lack the invariant chain associated with major histocompatibility complex (MHC) class II molecules with a reduction in surface class II molecules.

Detailed description

Mice homozygous for the Ii^tm1Liz targeted mutation are viable and fertile. The phenotype exhibited by mutant mice lacking the Ii (Ia-associated invariant chain) gene product is appreciably influenced by genetic background. As expected in BALB/cAn-Iitm1Liz mice, class II surface expression is markedly decreased. However, discordant expression of class II conformational epitopes is not detected, indicating that the few class II molecules present are properly folded and possess a normal configuration. Although spleen cells are ineffective for presentation of intact protein antigens, they show consistently superior peptide-loading capabilities. Thus surface class II molecules appear empty or occupied by easily displaced peptide(s). B lymphocyte development appears defective particularly in lymph node populations. Mature splenic dendritic cells in Ii chain mutants also display reduced class II surface expression. Thymi contain 3-fold fewer CD4+ T lymphocytes, but maturation of CD4+ T cells in the periphery occurs at near normal levels and secondary proliferative responses are elicited upon peptide challenge.

Allele

Symbol: Cd74^tm1Liz
Name: targeted mutation 1, Elizabeth K Bikoff
MGI accession ID: MGI:1927530
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd74
Marker name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Chromosome: 18
Strain of origin: 129S/SvEv-Gpi1^c
Molecular note: A MC1neopA cassette replaced the first intron and 11 nucleotides of the second exon.