These Cd74 (formerly Ii) knock-out mice lack the invariant chain associated with major histocompatibility complex (MHC) class II molecules with a reduction in surface class II molecules.
Dr. Elizabeth Bikoff, University of Oxford.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cd74 | CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) |
Mice homozygous for the Iitm1Liz targeted mutation are viable and fertile. The phenotype exhibited by mutant mice lacking the Ii (Ia-associated invariant chain) gene product is appreciably influenced by genetic background. As expected in BALB/cAn-Iitm1Liz mice, class II surface expression is markedly decreased. However, discordant expression of class II conformational epitopes is not detected, indicating that the few class II molecules present are properly folded and possess a normal configuration. Although spleen cells are ineffective for presentation of intact protein antigens, they show consistently superior peptide-loading capabilities. Thus surface class II molecules appear empty or occupied by easily displaced peptide(s). B lymphocyte development appears defective particularly in lymph node populations. Mature splenic dendritic cells in Ii chain mutants also display reduced class II surface expression. Thymi contain 3-fold fewer CD4+ T lymphocytes, but maturation of CD4+ T cells in the periphery occurs at near normal levels and secondary proliferative responses are elicited upon peptide challenge.
Allele Name | targeted mutation 1, Elizabeth K Bikoff |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ii-; Iitm1Liz; MHCII- |
Gene Symbol and Name | Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv-Gpi1c |
Chromosome | 18 |
Molecular Note | A MC1neopA cassette replaced the first intron and 11 nucleotides of the second exon. |
When maintaining a live colony, homozygous mice may be bred together. The expected coat color from breeding is albino.
When using the CD74- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002730 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Cd74<tm1Liz>, 1 pair minimum |
Frozen Mouse Embryo | CAn.129S(B6)-Cd74<tm1Liz>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | CAn.129S(B6)-Cd74<tm1Liz>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | CAn.129S(B6)-Cd74<tm1Liz>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | CAn.129S(B6)-Cd74<tm1Liz>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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