mammalian phenotype

• hematopoietic system phenotype
  • Normal - no evidence for anemia or thrombocytopenia
  (MGI Ref ID J:155870)

cellular phenotype

• decreased T cell proliferation
  • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
  (MGI Ref ID J:28966)

immune system phenotype

• abnormal T-helper 1 physiology
  • in vitro, lymph node cells from mice receiving wild-type MOG T cells exhibit reduced Th1 recall response compared with similarly treated wild-type mice
  (MGI Ref ID J:125654)
  • in vitro, the frequency of Th1 recall response is greater than in H2-Dma^tm1Luc homozygotes
  (MGI Ref ID J:125654)
  • in vivo, Th1 response to MOG is absent unlike in wild-type mice
  (MGI Ref ID J:125654)
  • in vivo, Th1 response to PLP is reduced compared to in wild-type mice
  (MGI Ref ID J:125654)

• decreased susceptibility to type IV hypersensitivity reaction
  • dramatically compared to in wild-type mice when wild-type MOG T cells are transferred
  (MGI Ref ID J:125654)

• abnormal antigen presentation
  • antigen presenting cells cannot process and present recombinant MOG protein unlike wild-type cells
  (MGI Ref ID J:125654)

• abnormal level of surface class II molecules
  • mutants expressed greatly reduced amounts of class II on spleen cells compared to wild-type
  (MGI Ref ID J:28966)

• abnormal antigen presentation via MHC class II
  • class II molecules are relatively ineffective for presentation of native protein antigens
  (MGI Ref ID J:28966)

• decreased susceptibility to experimental autoimmune encephalomyelitis
  • PLP-treated mice fail to develop EAE unlike similarly treated wild-type mice
  (MGI Ref ID J:125654)
  • whether MOG-primed or adoptively transferred with MOG-specific T blasts, mice fail to develop symptoms of experimental autoimmune encephalomyelitis (EAE) unlike wild-type mice
  (MGI Ref ID J:125654)

• defective assembly of class II molecules
  • lls; floppy conformers are seen, compared to compact dimers in wild-type
  (MGI Ref ID J:28966)
  • in spleen cells, immature alpha and beta chains exhibiting no evidence of having been exported past the cis-Golgi are observed, whereas compact alpha/beta dimers efficiently transported past the cis-Golgi are produced by Cd74^tm2Liz-deficient cells; floppy conformers are seen, compared to compact dimers in wild-type
  (MGI Ref ID J:28966)

• decreased CD4-positive, alpha beta T cell number
  • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
  (MGI Ref ID J:28966)

• decreased T cell proliferation
  • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
  (MGI Ref ID J:28966)

hematopoietic system phenotype

• abnormal T-helper 1 physiology
  • in vitro, lymph node cells from mice receiving wild-type MOG T cells exhibit reduced Th1 recall response compared with similarly treated wild-type mice
  (MGI Ref ID J:125654)
  • in vitro, the frequency of Th1 recall response is greater than in H2-Dma^tm1Luc homozygotes
  (MGI Ref ID J:125654)
  • in vivo, Th1 response to MOG is absent unlike in wild-type mice
  (MGI Ref ID J:125654)
  • in vivo, Th1 response to PLP is reduced compared to in wild-type mice
  (MGI Ref ID J:125654)

• decreased T cell proliferation
  • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
  (MGI Ref ID J:28966)

• decreased CD4-positive, alpha beta T cell number
  • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
  (MGI Ref ID J:28966)

hematopoietic system phenotype

• abnormal positive T cell selection
  • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow
  (MGI Ref ID J:125548)

immune system phenotype

• abnormal positive T cell selection
  • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow
  (MGI Ref ID J:125548)

mammalian phenotype

• hematopoietic system phenotype
  • Normal - no evidence for anemia or thrombocytopenia
  (MGI Ref ID J:155870)

hematopoietic system phenotype

• decreased CD4-positive, alpha beta T cell number
  • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold
  (MGI Ref ID J:110927)
  • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens
  (MGI Ref ID J:113714)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

immune system phenotype

• abnormal antigen presentation via MHC class II
  • mutant spleen cells on an H-2^d background show enhanced peptide-loading capabilities over wild-type cells
  (MGI Ref ID J:110927)
  • antigen presentation by mutant spleen cells to E^drestricted T cell clones is substantially decreased
  (MGI Ref ID J:113714)
  • spleen cells are ineffective for presentation of intact protein antigen as opposed to processed peptide antigens
  (MGI Ref ID J:110927)
  • T cell hybridomas produce Il-2 and T cell lines proliferate when stimulated by mutant spleen cells; similar results are seen using T cell clones specific for peptides including ovalbumin (OVA)_323-339 and IgG2a^b
  (MGI Ref ID J:110927)
  • E^drestricted T cells show enhanced activity when challenged with endogenously process beta 2 microglobulin (B2m) epitopes in presence of mutant splenocytes
  (MGI Ref ID J:113714)
  • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
  (MGI Ref ID J:110927)

• decreased CD4-positive, alpha beta T cell number
  • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold
  (MGI Ref ID J:110927)
  • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens
  (MGI Ref ID J:113714)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

• abnormal dendritic cell antigen presentation
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
  (MGI Ref ID J:110927)

• abnormal professional antigen presenting cell physiology
  • splenocytes display enhanced binding capabilities
  (MGI Ref ID J:113714)

cellular phenotype

• decreased T cell proliferation
  • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
  (MGI Ref ID J:72243)

immune system phenotype

• decreased susceptibility to experimental autoimmune encephalomyelitis
  • mice do not develop experimental autoimmune encephalitis (EAE) upon direct immunization with p35-55 while wild-type mice do develop EAE
  (MGI Ref ID J:72243)
  • adoptive transfer of wild-type encephalitogenic T cells does not induce EAE in mutant recipients
  (MGI Ref ID J:72243)

• defective assembly of class II molecules
  • majority of class II molecules are comprised of immature alpha and beta chains that have not been exported past the cis-Golgi
  (MGI Ref ID J:65918)
  • mutant spleen cells show significantly reduced amounts of alpha/beta heterodimers associated with the invariant chain; increased amounts of free alpha and beta chains are present
  (MGI Ref ID J:65918)
  • class II molecules produced by mutants show enhanced peptide binding compared to wild-type; molecules behave as if empty or occupied by easily displaced peptide in contrast to wild-type cells that are stably occupied with self and serum-derived peptides
  (MGI Ref ID J:65918)
  • no mature compact SDS-resistant alpha/beta heterodimers (I-A^b) are produced by mutant spleens in contrast to wild-type cells
  (MGI Ref ID J:65918)

• abnormal antigen presentation via MHC class II
  • mutant spleen cells stimulated stronger responses in presence of already processed peptides hen egg lysozyme (HEL) 74-88 and Ealpha 52-68, but fail to present native protein antigens keyhole limpet cyanin (KLH) and IgG2a
  (MGI Ref ID J:65918)

• decreased CD4-positive, alpha beta T cell number
  • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
  (MGI Ref ID J:65918)
  • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells
  (MGI Ref ID J:65918)

• decreased T cell proliferation
  • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
  (MGI Ref ID J:72243)

• abnormal antigen presentation
  • compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG
  (MGI Ref ID J:72243)
  • splenic antigen presenting cells (APCs) present an encephalitogenic MOG peptide p35-55 to I-A^b-restricted p35-55-specific T cells equivalently to wild-type APCs, stimulating proliferation but are ineffective at presenting intact MOG to T cells compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG
  (MGI Ref ID J:72243)

• abnormal level of surface class II molecules
  • mutant spleen cells exhibit reduced levels of I-A^b surface antigen compared to wild-type spleen cells
  (MGI Ref ID J:65918)

• decreased interferon-gamma secretion
  • immunization with p35-55 does not induce Ifng secretion indicating inability to prime p35-55 specific CD4+ T cells
  (MGI Ref ID J:72243)

growth/size/body region phenotype

• cachexia
  • occasionally, homozygotes display symptoms of severe wasting
  (MGI Ref ID J:65918)

• decreased body size
  • homozygotes are usually smaller than littermates
  (MGI Ref ID J:65918)

hematopoietic system phenotype

• decreased CD4-positive, alpha beta T cell number
  • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
  (MGI Ref ID J:65918)
  • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells
  (MGI Ref ID J:65918)

• decreased T cell proliferation
  • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
  (MGI Ref ID J:72243)

cellular phenotype

• abnormal T cell proliferation
  • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA_323-339 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

hematopoietic system phenotype

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

• abnormal T cell proliferation
  • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA_323-339 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

immune system phenotype

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

• abnormal antigen presentation via MHC class II
  • spleen cells from mice with an H-2^k haplotype background effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
  (MGI Ref ID J:110927)

• abnormal T cell proliferation
  • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA_323-339 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

• abnormal dendritic cell antigen presentation
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
  (MGI Ref ID J:110927)

cellular phenotype

• abnormal T cell proliferation
  • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL_46-61 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

hematopoietic system phenotype

• abnormal T cell proliferation
  • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL_46-61 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

immune system phenotype

• abnormal antigen presentation via MHC class II
  • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
  (MGI Ref ID J:110927)

• abnormal B cell differentiation
  • B cell maturation is defective
  (MGI Ref ID J:110927)

• abnormal dendritic cell antigen presentation
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
  (MGI Ref ID J:110927)

• abnormal T cell proliferation
  • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL_46-61 peptide show similar response to wild-type mice
  (MGI Ref ID J:110927)

• increased immature B cell number
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
  (MGI Ref ID J:110927)

hematopoietic system phenotype

• decreased B cell number
  • in the spleen and lymph node
  (MGI Ref ID J:194664)

immune system phenotype

• decreased B cell number
  • in the spleen and lymph node
  (MGI Ref ID J:194664)

immune system phenotype

• abnormal antigen presentation via MHC class II
  • mutants display isotype-specific antigen presentation defects; MHC A^k-restricted antigen presentation is reduced compared to wild-type
  (MGI Ref ID J:111565)