Cd74 knock-out mice lack the invariant chain associated with major histocompatibility complex (MHC) class II molecules with a reduction in surface class II molecules.
Dr. Elizabeth Bikoff, University of Oxford.
Mice homozygous for the Cd74tm1Liz targeted mutation (formerly called Iitm1Liz) are viable and fertile. Ia-associated invariant chain deficient mice have defects in MHC Class II assembly, transport, surface expression, and antigen presentation. Homozygous mutant mice lack mature CD4+ cells.
This strain was developed in the laboratory of Dr. Elizabeth Bikoff at Harvard University. The targeting vector carried the neomycin resistance gene.
|Allele Name||targeted mutation 1, Elizabeth K Bikoff|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ii-; Iitm1Liz; MHCII-|
|Gene Symbol and Name||Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||A MC1neopA cassette replaced the first intron and 11 nucleotides of the second exon.|
When maintaining a live colony, homozygous mice may be bred together. The expected coat color from breeding is black.
When using the CD74- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002729 in your Materials and Methods section.