Hemizygous mice for this transgene show increased levels of pre-B cells in the bone marrow throughout life and an increase in spontaneous pre-B and B cell lymphomas.
Dr. Alan Harris, The Walter & Eliza Hall Inst of Med Res
Expression of the mouse Myc transgene is restricted to the B cell lineage. Hemizygotes show increased pre-B cells in the bone marrow throughout life and a transient increase in large pre-B cells in the blood at 3-4 weeks of age. Spontaneous pre-B and B cell lymphomas reach an incidence of 50% at 15-20 weeks in hemizygous progeny of a wildtype female mated with a hemizygous male. The transgene synergizes with an TgN(BCL2)22Wehi transgene (Stock #002319) to produce primitive lymphoid tumors within 5 weeks of birth, and with an Emu-v-abl transgene to produce plasmacytomas by 8 weeks.
The TgN(IghMyc)22Bri transgenic strain was made in the laboratory of Dr Ralph Brinster, University of Pennsylvania in collaboration with Dr. Alan Harris, currently of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. The transgene construct consists of the Myc oncogene (c-myc) in association with the Emu immunoglobulin heavy chain enhancer and Myc promoter.
|Expressed Gene||Myc, myelocytomatosis oncogene, mouse, laboratory|
|Site of Expression|
|Allele Name||transgene insertion 22, Ralph L Brinster|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Allele Synonym(s)||E-myc; Emu-c-myc; Emu-cmyc Tg; Emu-myc; Eu-Myc; Tg-IghMyc-22Bri|
|Gene Symbol and Name||Tg(IghMyc)22Bri, transgene insertion 22, Ralph L Brinster|
|Gene Synonym(s)||Emu-c-myc; Emu-cmyc Tg; Emu-myc|
|Promoter||Myc, myelocytomatosis oncogene, mouse, laboratory|
|Expressed Gene||Myc, myelocytomatosis oncogene, mouse, laboratory|
|Strain of Origin||(C57BL x SJL)F2|
|General Note||13 out of 15 transgenic founders developed lymphomas between 6 and 15 weeks of age. The animals became terminally ill and died or were sacrificed. |
Hemizygous mice on a C57BL/6 background display increased pre-B cells in the bone marrow throughout life and a transient increase in large pre-B cells in the blood at 3-4 weeks of age. Spontaneous pre-B and B cell lymphomas reach an incidence of 50% at 15-20 weeks in hemizygous progeny of a wildtype female mated with a hemizygous male.
In conjuction with Tg(BCL2)22Wehi, transgenic mice on a C57BL/6 background exhibit the production of primitive lymphoid tumors within 5 weeks of birth
|Molecular Note||Expression of the mouse Myc transgene is directed to the B cell lineage by the Igh (E mu) enhancer and Myc promoter. The transgene was originally designated Eμ-Myc.|
The strain is maintained by mating hemizygous male to a wildtype C57BL/6J female.
|Please inquire about possible genotypes.|
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