B6;129-Ahr^tm1Bra/J

Stock number: 002727
Product name: Ahr null
Research areas: Cancer Research, Developmental Biology Research, Hematological Research, Internal/Organ Research, Metabolism Research, Research Tools

Description

These Ahr knock-out mice exhibit hepatic defects.

Detailed description

Mice homozygous for the Ahr^tm1Bra targeted mutation are viable and fertile. Homozygotes do not respond to aryl-hydrocarbon receptor agonists. They show reduced liver weight (25% decrease) delayed extramedullary hematopoiesis, and transient hepatic microvesicular steatosis.

Development

The strain was developed using a construct that replaced exon 2 of the endogenous gene with the neomycin gene. The construct was electroporated into both (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells and CCE-derived 129S6/SvEvTac ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and transfered into pseudopregnant CD1 foster mothers. Chimeric males were bred to C57BL/6 females. Heterozygous mice were bred to generate homozygotes.

Allele

Symbol: Ahr^tm1Bra
Name: targeted mutation 1, Christopher A Bradfield
MGI accession ID: MGI:1857427
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ahr
Marker name: aryl-hydrocarbon receptor
Chromosome: 12
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin selection cassette replaced a genomic fragment containing exon 2, which encodes the basic-HLH domain essential for dimerization and DNA binding. Western blot analysis on liver cytosol demonstrated that the protein was not detectable in homozygous mice.