B6.129-Ptprc^tm1Holm/J

Stock number: 002725
Product name: D45 null
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

Mice homozygous for the Ptprc^tm1Holm targeted mutation are viable and fertile. Homozygous mutant mice have reduced numbers of peripheral T cells, remaining T cells are defective. Mice are severely immunodeficient and vulnerable to infection.

Allele

Symbol: Ptprc^tm1Holm
Name: targeted mutation 1, Nick Holmes
MGI accession ID: MGI:1857277
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ptprc
Marker name: protein tyrosine phosphatase, receptor type, C
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin resistance cassette, corrected for a point mutation believed to affect the transformation efficiency of ES cells, was used to disrupt exon 9, which is common to all of the isoforms. The mutation results in the introduction of several stop codons downstream of the insertion. FACS analysis using antibodies for multiple isoforms showed an absence of cell surface expression in homozygous mutant mice. A lack of encoded protein was further confirmed by Western blot analysis of thymus lysates.