These Il12a knock-out mice exhibit an inability to mount a delayed-type hypersensitivity (DTH) reaction, but a T-helper 2 cell (TH2) response is induced.
Dr. Jeanne Magram, Celsius Therapeutics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il12a | interleukin 12a |
Mice homozygous for the Il12atm1Jm targeted mutation on a normally resistant 129/Sv genetic background are unable to restrict the progression of Leishmania major infection, demonstrating the importance of this cytokine for developing such resistance. Homozygotes are unable to mount a delayed-type hypersensitivity (DTH) reaction but a TH2 response is induced. Also known as p35. Please refer to the Il12b-deficient related targeted mutations (Stock No. 002693 and 002694). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The targeting strategy removed exons 1 and 2 from the Il12a gene.
Allele Name | targeted mutation 1, Jeanne Magram |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Il12a-; IL-12p35; IL-12p35-; Il12p35-; p35- |
Gene Symbol and Name | Il12a, interleukin 12a |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 3 |
Molecular Note | A neomycin resistance cassette replaced exons 1 and 2 of the gene. |
Mutations Made By | Dr. Jeanne Magram, Celsius Therapeutics |
This strain is maintained by homozygous sibling matings. Expected coat color from breeding:Albino
When using the Il-12p35 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002691 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Il12a<tm1Jm> |
Frozen Mouse Embryo | C.129S1(B6)-Il12a<tm1Jm>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S1(B6)-Il12a<tm1Jm>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S1(B6)-Il12a<tm1Jm>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.129S1(B6)-Il12a<tm1Jm>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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