C.129S1(B6)-Il12a^tm1Jm/J

Stock number: 002691
Product name: Il-12p35 KO
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Il12a knock-out mice exhibit an inability to mount a delayed-type hypersensitivity (DTH) reaction, but a T-helper 2 cell (TH2) response is induced.

Detailed description

Mice homozygous for the Il12a^tm1Jm targeted mutation on a normally resistant 129/Sv genetic background are unable to restrict the progression of Leishmania major infection, demonstrating the importance of this cytokine for developing such resistance. Homozygotes are unable to mount a delayed-type hypersensitivity (DTH) reaction but a TH2 response is induced. Also known as p35. Please refer to the Il12b-deficient related targeted mutations (Stock No. 002693 and 002694). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The targeting strategy removed exons 1 and 2 from the Il12a gene.

Allele

Symbol: Il12a^tm1Jm
Name: targeted mutation 1, Jeanne Magram
MGI accession ID: MGI:1857200
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il12a
Marker name: interleukin 12a
Chromosome: 3
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A neomycin resistance cassette replaced exons 1 and 2 of the gene.