These mice carry a targeted duplication mutation at the Agt locus characterized by elevated blood pressure.
Dr. Oliver Smithies, University of North Carolina at Chapel Hill
The entire agiotensinogen gene (Agt) was duplicated by homologous recombination allowing the examination of the effects of gene copy number on normal AGT function. Homozygous mice (Agtdup/Agtdup) are viable and fertile. They show blood pressure levels ~16 mmHg higher than normal wildtype siblings (two copies of Agt gene). Hemizygous mice (Agtdup/+) show blood pressure levels ~8 mmHg higher than normal wildtype siblings. This transgenic demonstrates the causality between genotypes at the angiotensinogen locus and blood pressures. Blood pressures of one-copy (Agttm1Unc/+) through four-copy (Agtdup/Agtdup) animals show significant and almost linear increases of approximately 8mmHg per gene copy despite their normal compensatory mechanisms being intact.
|Allele Name||targeted mutation 2, University of North Carolina|
|Allele Type||Targeted (Not Applicable)|
|Allele Synonym(s)||AgtTgH(Agtdup)1Unc; Tg(Agtdup)1Unc; TgH(Agtdup)1Unc|
|Gene Symbol and Name||Agt, angiotensinogen (serpin peptidase inhibitor, clade A, member 8)|
|Promoter||Agt, angiotensinogen (serpin peptidase inhibitor, clade A, member 8), mouse, laboratory|
|Strain of Origin||129P2/OlaHsd|
|General Note||Human AGT and renin genes can complement the effects of the mouse null mutation (J:39934).|
|Molecular Note||A tandem duplication of the gene was created by "gap-repair" gene targeting. Radioimmunoassay tests demonstrated increased plasma levels of the encoded protein in heterozygous mice.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Agt duplication mouse strain in a publication, please cite the originating article(s) and include JAX stock #002690 in your Materials and Methods section.