B6.129P2-Agtr1a^tm1Unc/J

Stock number: 002682
Product name: AT1AR KO
Strain synonyms: B6.129P2-Agtr1^tm1Unc/J
Research areas: Cardiovascular Research

Description

Homozygous mice show no response to angiotensin II infusion and have lower blood pressure than normal wildtype siblings. Heterozygous mice have an altered response to angiotensin II infusion and lower blood pressure.

Detailed description

Mice homozygous mice for the targeted mutation are viable and fertile. Pressor responses to angiotensin II infusions are absent and blood pressure levels are ~24 mmHg lower than normal wildtype siblings. Heterozygous mice have blood pressure levels ~12 mmHg lower than normal wildtype siblings and show qualitatively altered responses to angiotensin II infusions.

Development

Coding sequences from the emdogenous gene were disrupted by insertion of a neomycin resistance gene construct. The targeting construct was electroporated into 129P2/OlaHsd-derived BK4 (E14TG2a clone)embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric males were bred with C57BL/6J females. This mutant was backcrossed to C57BL/6 at least seven generations.

Allele

Symbol: Agtr1a^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857147
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Agtr1a
Marker name: angiotensin II receptor, type 1a
Chromosome: 13
Strain of origin: 129P2/OlaHsd
Molecular note: A 0.5kb genomic fragment containing part of the coding sequences of the gene was replaced with a neomycin selection cassette. Binding studies in kidney indicated that no functional protein was detectable in homozygous mice.
General note: Kidneys of these null receptor mice are normal except for some expansion of renin-producing cells (J:45507).