Homozygous mice show no response to angiotensin II infusion and have lower blood pressure than normal wildtype siblings. Heterozygous mice have an altered response to angiotensin II infusion and lower blood pressure.
Dr. Oliver Smithies, University of North Carolina at Chapel Hill
Mice homozygous mice for the targeted mutation are viable and fertile. Pressor responses to angiotensin II infusions are absent and blood pressure levels are ~24 mmHg lower than normal wildtype siblings. Heterozygous mice have blood pressure levels ~12 mmHg lower than normal wildtype siblings and show qualitatively altered responses to angiotensin II infusions.
Coding sequences from the emdogenous gene were disrupted by insertion of a neomycin resistance gene construct. The targeting construct was electroporated into 129P2/OlaHsd-derived BK4 (E14TG2a clone)embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric males were bred with C57BL/6J females. This mutant was backcrossed to C57BL/6 at least seven generations.
|Allele Name||targeted mutation 1, University of North Carolina|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Agt1ar-; Agtr1tm1Unc; Agtr1A-; At1A-; AT1A-KO; AT1AR KO; AT1aR-; AT1-R-|
|Gene Symbol and Name||Agtr1a, angiotensin II receptor, type 1a|
|Strain of Origin||129P2/OlaHsd|
|General Note||Kidneys of these null receptor mice are normal except for some expansion of renin-producing cells (J:45507).|
|Molecular Note||A 0.5kb genomic fragment containing part of the coding sequences of the gene was replaced with a neomycin selection cassette. Binding studies in kidney indicated that no functional protein was detectable in homozygous mice.|
When maintaining a live colony, homozygous mice are bred. Expected coat color from breeding: Black.
When using the AT1AR KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002682 in your Materials and Methods section.