These Ace knock-out mice exhibit show reduced viability prior to weaning and reduced blood pressure in adults. They are suitable for use in applications related to the study of hypertension.
Dr. Oliver Smithies, University of North Carolina at Chapel Hill
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ace | angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 |
Mice homozygous for the Acetm1Unc targeted mutation lack both the somatic and testicular isozyme forms of the Ace gene. They show reduced viability prior to weaning. Surviving homozygotes are hypotensive with blood pressures ~35 mmHg lower than normal wildtype silbings. Heterozygous male mice have blood pressures 15-20 mmHg below wildtype siblings. Fertility of homozygous males is greatly impaired. Ten to twelve month old female homoygotes exhibit abnormal renal vessels and tubules, increased renin synthesis accompanied by an abnormal expression pattern.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ACE-KO; stst |
Gene Symbol and Name | Ace, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A neomycin selection cassette was inserted into exon 14 of the gene. Exon 14 encodes amino acids critical for the normal function of both isoforms of the protein. |
Mutations Made By | Dr. Oliver Smithies, University of North Carolina at Chapel Hill |
This strain was generated on a 129/Ola genetic background. As of 5/96 it had been backcrossed 8 times to C57BL/6J. It is maintained by breeding heterozygous males to C57BL/6J females. The Y chromosome in these males is therefore 129/Ola, ES cell-derived. 11/6/98: Contact Peter Schweitzer for typing information. Expected coat color from breeding:Black
When using the Act zero copy mouse strain in a publication, please cite the originating article(s) and include JAX stock #002679 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Ace<tm1Unc> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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