FVB.Cg-Tg(WapTgfa)215Bri/J

Stock number: 002678
Product name: WAP-TGF-α line 3573-2
Research areas: Cancer Research

Description

These WAP-TGF-a line 3573-2 mice express a transgene containing the mouse transforming growth factor alpha gene directed by the mouse whey acidic protein promoter.

Detailed description

Transgenic females showed precocious mammary gland development during pregnancy. Involution was delayed beyond 8 days post-parturition. With successive pregnancies hyperplastic alveolar nodules increased in number. Mammary tumors, the majority of which were well-differentiated, were observed with variable latency, on average at 7-8 months of age in multiparious females. Fibroadenomas composed of neoplastic glandular alveolar epithelium surrounded by variable amounts of stroma were also observed. Metastasis to the lung was observed in 1 of 40 tumor-bearing mice. Virgin mice were tumor-free.

Development

Transgene constructs were prepared by inserting a 0.7 kb c DNA fragment that encodes the entire rat transforming growth factor alpha (Tgfa) gene into a KpnI site in the first exon of the mouse whey acidic protein (Wap) gene. This strain was originally generated on a B6SJLF1 genetic background. It has been backcrossed 8 times to FVB/N.

Allele

Symbol: Tg(WapTgfa)215Bri
Name: transgene insertion 215, Ralph L Brinster
MGI accession ID: MGI:3054253
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(WapTgfa)215Bri
Marker name: transgene insertion 215, Ralph L Brinster
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: Tgfa,transforming growth factor alpha,rat
Molecular note: The transgene contains the mouse whey acidic protein (WAP) promoter and the mouse transforming growth factor alpha (Tgfa) gene.