B6.PERA-Tnfsf4^{Ath1-PERA/EiJ}/Pgn

Stock number: 002671
Product name: B6.PERA-Ath1^r
Research areas: Cardiovascular Research

Detailed description

This strain carries the Tnfsf4^{Ath1-PERA/EiJ} resistance allele of the wild-derived strain PERA, which confers a high serum HDL cholesterol level and resistance to the development of atherosclerosis when the mice are fed a high fat diet on the C57BL/6J strain background. The C57BL/6J allele results in low HDL cholesterol levels and susceptibility to diet induced atherosclerosis.

Development

Mice of the strain PERA, descended from wild mice trapped in Peru, are resistant to atherosclerosis and have elevated HDL levels. A congenic strain was constructed by crossing a PERA mouse to a C57BL/6J mouse, then selecting mice having high HDL levels for each subsequent backcross to C57BL/6J. At N5, the high HDL/ atherosclerosis resistance trait was mapped and found to localize to the same genomic position as Ath1. The congenic has now been backcrossed for ten generations. (The PERA subline used to construct this strain is now extinct.) (Paigen, 2000)

B6.PERA-Tnfsf4^{Ath1-PERA/EiJ} was transfered to the production colony at JAX from Dr. Beverly Paigen's colony, also at JAX. The PERA/Ei inbred used as the donor strain in this congenic originally came from Peru. The original was trapped by Olga Atteck for M.E. Wallace, University of Cambridge, in 1961 in a Peruvian Yard used to dry maize cobs in Nana Village, Rimac Valley, Peru. The mice were held in a small, closed colony by Wallace. In 1971, at approximately F25, mice from his colony were sent to E.M. Eicher and T.H. Roderick at JAX. PERA/Ei and PERA/Rk were established immediatelly as separate colonies.

Allele

Symbol: Tnfsf4^{Ath1-PERA/EiJ}
Name: PERA/EiJ
MGI accession ID: MGI:3576370
Generation method: QTL
Marker symbol: Tnfsf4
Marker name: tumor necrosis factor (ligand) superfamily, member 4
Chromosome: 1
Strain of origin: PERA/EiJ
Molecular note: This allele confers elevated high-density lipoprotein (HDL) cholesterol levels and resistance to diet-induced atherosclerosis relative to the C57BL/6J allele.