These Cd28 knock-out mice exhibit an impaired response to lectins. They are suitable for use in applications related to the study of cell-mediated and humoral immune responses in vivo.
Dr. Tak Mak, University Health Network/Un of Toronto
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd28 | CD28 antigen |
Mice homozygous for the Cd28tm1Mak targeted mutation exhibit normal development of T and B cells, but have an impaired response to lectins. Other characteristics of homozygous mutant mice include decreased IL2 receptor expression, reduced basal Ig levels, low titers of IgG1 and IgG2b but increased IgG2a, reduced activity of T helper cells and diminished Ig class switching after infection with vesicular stomatitis virus. Infection of mice with lymphocytic choriomeningities virus creates induced and delayed-type hypersensitivity response in cytotoxic T cells.
Allele Name | targeted mutation 1, Tak Mak |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD28-; CD28KO |
Gene Symbol and Name | Cd28, CD28 antigen |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A genomic fragment containing part of exon 2 was replaced by a neomycin resistance cassette. Flow-cytometry analysis on peripheral blood lymphocytes demonstrated that the protein was not expressed in these cells in homozygous mice. |
Mutations Made By | Dr. Tak Mak, University Health Network/Un of Toronto |
When maintaining a live colony, these mice can be bred as homozygotes. The expected coat color from breeding is albino.
When using the CD28- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002667 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Cd28<tm1Mak> |
Frozen Mouse Embryo | C.129S2(B6)-Cd28<tm1Mak>/J | $2595.00 |
Frozen Mouse Embryo | C.129S2(B6)-Cd28<tm1Mak>/J | $2595.00 |
Frozen Mouse Embryo | C.129S2(B6)-Cd28<tm1Mak>/J | $3373.50 |
Frozen Mouse Embryo | C.129S2(B6)-Cd28<tm1Mak>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.