C.129S2(B6)-Cd28^tm1Mak/J

Stock number: 002667
Product name: CD28^-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Cd28 knock-out mice exhibit an impaired response to lectins. They are suitable for use in applications related to the study of cell-mediated and humoral immune responses in vivo.

Detailed description

Mice homozygous for the Cd28^tm1Mak targeted mutation exhibit normal development of T and B cells, but have an impaired response to lectins. Other characteristics of homozygous mutant mice include decreased IL2 receptor expression, reduced basal Ig levels, low titers of IgG1 and IgG2b but increased IgG2a, reduced activity of T helper cells and diminished Ig class switching after infection with vesicular stomatitis virus. Infection of mice with lymphocytic choriomeningities virus creates induced and delayed-type hypersensitivity response in cytotoxic T cells.

Allele

Symbol: Cd28^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1857150
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd28
Marker name: CD28 antigen
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: A genomic fragment containing part of exon 2 was replaced by a neomycin resistance cassette. Flow-cytometry analysis on peripheral blood lymphocytes demonstrated that the protein was not expressed in these cells in homozygous mice.