B6;129S-Cd4^tm1Mak Cd8a^tm1Mak/J

Stock number: 002664
Product name: CD4- CD8-
Research areas: Immunology, Inflammation and Autoimmunity Research

Detailed description

Mice homozygous for both the Cd4^tm1Mak and Cd8a^tm1Mak targeted mutations have a significant block in both CD4⁺ and CD8⁺ T-cell development. Homozygous mutant mice also show a Class II restricted deficit in helper T-cell activity and other T-cell responses.

Allele

Symbol: Cd4^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1857144
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd4
Marker name: CD4 antigen
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette was inserted into exon 5. Flow cytometry analysis demonstrated that the protein was absent from the cell surface of thymocytes and lymph node cells in homozygous mice.

Allele

Symbol: Cd8a^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1857149
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd8a
Marker name: CD8 antigen, alpha chain
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance gene was inserted into exon 1. Flow cytometry analysis on thymus and lymph node cells derived from homozygous mice confirmed that no detectable encoded protein was expressed on the cell surface.