Mice homozygous for the Cd4tm1Mak targeted mutation have a significant block in CD4+ T-cell development. Cell surface expression of CD4 protein is not detected on thymocytes and lymph node cells from homozygous mice. Homozygous mutant mice also show a Class II restricted deficit in helper T-cell activity and other T-cell responses. This mutant mouse strain may be useful in studies of T cell development, susceptibility to viral infection and inflammation.
Dr. Tak Mak, University Health Network/Un of Toronto
Mice homozygous for the Cd4tm1Mak targeted mutation have a significant block in CD4+ T-cell development; 90% of their circulating T-cells are CD8+. Cell surface expression of CD4 protein is not detected on thymocytes and lymph node cells from homozygous mice. Homozygous mutant mice also show a Class II restricted deficit in helper T-cell activity and other T-cell responses. This mutant mouse strain may be useful in studies of T cell development, susceptibility to viral infection and inflammation.
A targeting vector containing a PGK-neo cassette was used to disrupt exon 5. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to C57BL/6 mice for 8 generations prior to arrival. As of 2018, this colony has been backcrossed to C57BL/6 for a total of at least 10 generations.
|Allele Name||targeted mutation 1, Tak Mak|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD4-; CD4 KO; CD40|
|Gene Symbol and Name||Cd4, CD4 antigen|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance cassette was inserted into exon 5. Flow cytometry analysis demonstrated that the protein was absent from the cell surface of thymocytes and lymph node cells in homozygous mice.|
|Mutations Made By|| |
Dr. Tak Mak, University Health Network/Un of Toronto
When maintaining a live colony, these mice can be bred as homozygotes. Expected coat color from breeding is black.
When using the CD4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002663 in your Materials and Methods section.
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