B6;SJL-Tg(EPO33)72Ptc/J

Stock number: 002661
Product name: Epo33

Description

These EP033 mice express a transgene containing a fragment of the human erythropoietin gene.

Detailed description

Homozygotes are viable and fertile. The transgene in line Epo33-72 encompasses a 33 kb genomic fragment including 4 kb of human EPO along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Anemia produced by phelebotomy or treatment with phenylhydrazine induces the transgene in kidney and liver with some variability. Cobalt and hypoxia also reportedly induce the transgene. Brain, heart, kidney, spleen and liver were tested in animals which did not have induced anemia and constitutive expression was not found.

Allele

Symbol: Tg(EPO33)72Ptc
Name: transgene insertion 72, Peter Curtin
MGI accession ID: MGI:3054249
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(EPO33)72Ptc
Marker name: transgene insertion 72, Peter Curtin
Chromosome: UN
Strain of origin: C57BL/6J x SJL/J
Expressed genes: EPO,erythropoietin,human
Molecular note: The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created. Gene copy number in line 72 is 2.
General note: Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver.