These EP033 mice express a transgene containing a fragment of the human erythropoietin gene.
Dr. Peter Curtin, New England Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Homozygotes are viable and fertile. The transgene in line Epo33-72 encompasses a 33 kb genomic fragment including 4 kb of human EPO along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Anemia produced by phelebotomy or treatment with phenylhydrazine induces the transgene in kidney and liver with some variability. Cobalt and hypoxia also reportedly induce the transgene. Brain, heart, kidney, spleen and liver were tested in animals which did not have induced anemia and constitutive expression was not found.
Expressed Gene | EPO, erythropoietin, human |
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Site of Expression |
Allele Name | transgene insertion 72, Peter Curtin |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(EPO33)72Ptc, transgene insertion 72, Peter Curtin |
Gene Synonym(s) | |
Promoter | Ucp, mitochondrial uncoupling protein, |
Expressed Gene | EPO, erythropoietin, human |
Strain of Origin | C57BL/6J x SJL/J |
Chromosome | UN |
General Note | Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver. |
Molecular Note | The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created. Gene copy number in line 72 is 2. |
Mutations Made By | Dr. Peter Curtin, New England Medical Center |
When using the Epo33 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002661 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(EPO33)72Ptc |
Frozen Mouse Embryo | B6;SJL-Tg(EPO33)72Ptc/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(EPO33)72Ptc/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(EPO33)72Ptc/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(EPO33)72Ptc/J Frozen Embryo | $3373.50 |
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