FVB/N-Tg(Trp53R172H)8512Jmr/J

Stock number: 002659
Product name: p53 172^R-H
Research areas: Cancer Research

Description

These p53 172^R-H mice express a transgene containing an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172, and exhibit a shorter latency of developed tumors after treatment with the carcinogen dimethylbenz(a)anthracene.

Detailed description

The FVB/NTgN(Trp53R172H)8512Jmr express TRP53 with both dominant-negative and a gain-of function properties, i.e. this mutant is capable of inducing multiple drug resistance(MDR) promoter-driven reporter gene expression in transfection studies performed in p53 null cells. Transgene expression alone exerts no apparent effect on normal mammary gland development. Mice treated with the chemical carcinogen, dimethylbenz(a)anthracine (DMBA) or crossed with mice overexpressing erb-B2 develop tumors with significantly shorter latencies than controls. These tumors are characterized by large pleiomorphic nuclei and genomic instability. Spontaneous tumors are rarely observed in multiply bred animals in the first year of life.

Development

0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)

Allele

Symbol: Tg(Trp53R172H)8512Jmr
Name: transgene insertion 8512, Jeffrey Rosen
MGI accession ID: MGI:1929642
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Trp53R172H)8512Jmr
Marker name: transgene insertion 8512, Jeffrey Rosen
Chromosome: UN
Strain of origin: FVB
Expressed genes: Trp53,transformation related protein 53,mouse, laboratory
Molecular note: This transgene contains the rat whey acidic protein promoter and an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172 (CGC->CAC; Arg172His).
General note: On an FVB/N background transgenic mice express TRP53 with both dominant-negative and a gain-of function properties.