These p53 172R-H mice express a transgene containing an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172, and exhibit a shorter latency of developed tumors after treatment with the carcinogen dimethylbenz(a)anthracene.
Dr. Jeffrey Rosen, Baylor College of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
The FVB/NTgN(Trp53R172H)8512Jmr express TRP53 with both dominant-negative and a gain-of function properties, i.e. this mutant is capable of inducing multiple drug resistance(MDR) promoter-driven reporter gene expression in transfection studies performed in p53 null cells. Transgene expression alone exerts no apparent effect on normal mammary gland development. Mice treated with the chemical carcinogen, dimethylbenz(a)anthracine (DMBA) or crossed with mice overexpressing erb-B2 develop tumors with significantly shorter latencies than controls. These tumors are characterized by large pleiomorphic nuclei and genomic instability. Spontaneous tumors are rarely observed in multiply bred animals in the first year of life.
0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)
Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
---|---|
Site of Expression |
Allele Name | transgene insertion 8512, Jeffrey Rosen |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 172R-H; p53(R172H); p53-172H; Wap-p53R172H |
Gene Symbol and Name | Tg(Trp53R172H)8512Jmr, transgene insertion 8512, Jeffrey Rosen |
Gene Synonym(s) | |
Promoter | Wap, whey acidic protein, rat |
Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
General Note | On an FVB/N background transgenic mice express TRP53 with both dominant-negative and a gain-of function properties. |
Molecular Note | This transgene contains the rat whey acidic protein promoter and an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172 (CGC->CAC; Arg172His). |
Mutations Made By | Dr. Jeffrey Rosen, Baylor College of Medicine |
There are no reproductive or lactational defects observed in this strain so it can be maintained by breeding either FVB/NJ females or males. Expected coat color from breeding:Albino
When using the p53 172R-H mouse strain in a publication, please cite the originating article(s) and include JAX stock #002659 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Trp53R172H)8512Jmr |
Frozen Mouse Embryo | FVB/N-Tg(Trp53R172H)8512Jmr/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Trp53R172H)8512Jmr/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Trp53R172H)8512Jmr/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(Trp53R172H)8512Jmr/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.