These interleukin 6 (IL6) mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
IMR Colony, The Jackson Laboratory
Mice homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of lipopolysaccharide (LPS) challenged macrophages. Bioassay and enzyme-linked immunosorbent assay (ELISA) analysis of serum from LPS-challenged homozygotes reveals no detectable protein activity. These interleukin 6 (IL6) mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
Of note, IL6-mutant mice may be available on different genetic backgrounds including mixed B6;129S2 (Stock No. 002254), C57BL/6J (Stock No. 002650), BALB/cByJ (Stock No. 007078), and NOD/ShiLtJ (Stock No. 029264).
The interleukin 6 targeted mutation was created by Manfred Kopf and Georges Kohler (Max Planck Institut Fur Immunbiologie, Freiburg Germany). A targeting vector was designed to place a neomycin resistance cassette into the first coding exon (exon 2) of the targeted gene. This construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting mice were sent to The Jackson Laboratory where the mouse line was bred to C57BL/6J (Stock No. 000664) mice for 11 generations.
|Allele Name||targeted mutation 1, Manfred Kopf|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-6-; Il6-; IL-6 KO; Il6tm1Koe; Il6tm1Kopf|
|Gene Symbol and Name||Il6, interleukin 6|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin selection cassette was inserted into exon 2. RT-PCR experiments on RNA derived from LPS-stimulated macrophages of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA and bioassay experiments confirmed that no functional protein was made in homozygous mice.|
|Mutations Made By|| |
Dr. Manfred Kopf, ETH Zurich
This strain is maintained by mating homozygous siblings. The expected coat color from breeding is Black.
When using the Il-6 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002650 in your Materials and Methods section.