B6SJL-Tg(WTbeta2)4Wjk/J

Stock number: 002638
Product name: TG4

Description

These TG4 mice express a transgene that contains the human beta-2 adrenergic receptor (β₂-AR).

Detailed description

These transgenic mice exhibit increased basal myocardial adenylyl cyclase activity, enhanced atrial contractility, and increased left ventricular function in vivo; these parameters at baseline in the transgenic animals were equal to those observed in control animals maximally stimulated with isoproterenol. Mice carrying this transgene display a marked overexpression of the human beta-2 adrenergic receptor (~200 fold increase).

Development

This strain was developed in the laboratory of Dr. Walter Koch at Duke University Medical Center. The transgene consists of the human beta-2 adrenergic receptor, a SV40 introl/polyA sequence used for screening of mice, and the murine alpha-myosin heavy chain promoter. Transgenic mice were created with cardiac-specific overexpression of the Beta2 adrenergic receptor.

Allele

Symbol: Tg(WTbeta2)4Wjk
Name: transgene insertion 4, Walter Koch
MGI accession ID: MGI:3037368
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(WTbeta2)4Wjk
Marker name: transgene insertion 4, Walter Koch
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: ADRB2,adrenoceptor beta 2,human
Molecular note: The transgene consists of the human beta-2 adrenergic receptor (ADRB2), an SV40 intron/polyA sequence, and the murine myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter which directs cardiac-specific overexpression of ADRB2. Transgene expression is detected in cardiac myocytes at levels 200 times greater than in human cardiac tissue.