These CAM7 mice carry the CAMalpha1b transgene and display stable myocardial specific hypertrophy with ensuing markers of hypertrophy being elevated. In addition, alpha 1 adrenergic receptor signaling through the Gq-phospholipase C-protein kinase C signaling pathway is enhanced.
Dr. Walter Koch, Duke University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Constitutively active, Inserted expressed sequence) |
These CAM7 mice carry the CAMalpha1b transgene and display stable myocardial specific hypertrophy with ensuing markers of hypertrophy being elevated. In addition, alpha 1 adrenergic receptor signaling through the Gq-phospholipase C-protein kinase C signaling pathway is enhanced.
This strain was developed in the laboratory of Dr. Walter Koch at Duke University Medical Center. The transgene consists of a constitutively active form of the hamster adrenergic receptor, alpha 1b containing 3 missense mutations (R288K, K290H, A293L), a SV40 introl/polyA sequence used for screening of mice, and the murine alpha-myosin heavy chain promoter. This strain was generated on a B6SJLF1/J genetic background. It is currently on a mixed C57BL/6 x SJL genetic background.
Expressed Gene | ADRA1B, alpha1B-adrenergic receptor , hamster |
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Site of Expression |
Allele Name | transgene insertion 7, Walter Koch |
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Allele Type | Transgenic (Constitutively active, Inserted expressed sequence) |
Allele Synonym(s) | (CAM)alpha1B-AR; CAM7; CAMalpha1B-AR |
Gene Symbol and Name | Tg(CAMalpha1b)7Wjk, transgene insertion 7, Walter Koch |
Gene Synonym(s) | |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory |
Expressed Gene | ADRA1B, alpha1B-adrenergic receptor , hamster |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | Sequence encoding hamster adrenergic receptor alpha 1b (ADRA1B), containing 3 missense mutations (R288K, K290H, A293L) to make it constitutively active, was adjoined to the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Cardiac-specific expression of the mutant ADRA1B was indicated by radio ligand binding assay to be approximately 3-fold higher than that of the endogenous Adra1b. In addition to line 7 (CAM7), lines 21 (CAM21) and 56 (CAM56) were also generated. They also overexpress by approximately 3-fold. |
Mutations Made By | Dr. Walter Koch, Duke University Medical Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype mice from the colony.
When using the CAM7 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002637 in your Materials and Methods section.
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Carrier or non-carrier for Tg(CAMalpha1b)7Wjk |
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