B6SJL-Tg(CAMalpha1b)7Wjk/J

Stock number: 002637
Product name: CAM7

Detailed description

These CAM7 mice carry the CAMalpha1b transgene and display stable myocardial specific hypertrophy with ensuing markers of hypertrophy being elevated. In addition, alpha 1 adrenergic receptor signaling through the Gq-phospholipase C-protein kinase C signaling pathway is enhanced.

Development

This strain was developed in the laboratory of Dr. Walter Koch at Duke University Medical Center. The transgene consists of a constitutively active form of the hamster adrenergic receptor, alpha 1b containing 3 missense mutations (R288K, K290H, A293L), a SV40 introl/polyA sequence used for screening of mice, and the murine alpha-myosin heavy chain promoter. This strain was generated on a B6SJLF1/J genetic background. It is currently on a mixed C57BL/6 x SJL genetic background.

Allele

Symbol: Tg(CAMalpha1b)7Wjk
Name: transgene insertion 7, Walter Koch
MGI accession ID: MGI:1858099
Generation method: Transgenic
Attributes: Constitutively active; Inserted expressed sequence
Marker symbol: Tg(CAMalpha1b)7Wjk
Marker name: transgene insertion 7, Walter Koch
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: ADRA1B,alpha1B-adrenergic receptor ,hamster
Molecular note: Sequence encoding hamster adrenergic receptor alpha 1b (ADRA1B), containing 3 missense mutations (R288K, K290H, A293L) to make it constitutively active, was adjoined to the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Cardiac-specific expression of the mutant ADRA1B was indicated by radio ligand binding assay to be approximately 3-fold higher than that of the endogenous Adra1b. In addition to line 7 (CAM7), lines 21 (CAM21) and 56 (CAM56) were also generated. They also overexpress by approximately 3-fold.