Nos1 KO mice may useful when studying structure and function of neurons and their synaptic connections in the brain and periphery.
Dr. Paul Huang, Mass General Hospital, Harvard Med Sch
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nos1 | nitric oxide synthase 1, neuronal |
The nitric oxide signaling molecule produced by the neuronal Nos1 gene is widely expressed, affecting diverse systems. The knockout of this gene has been shown to affect such normal characteristics as cognition and behavior, long-term memory, cortical development, colonic motility, skeletal muscle contraction, ovarian cycle regulation, cardiac/cardiovascular function, and even sensitivity to Anthrax lethal toxin. Heterozygous mice are viable and fertile, while some lethality is seen in homozygotes due to stomach hypertrophy. Homozygotes have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer (Huang et al., 1993). Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. Homozygous males are reported to be more aggressive than normal wildtype siblings although this has not been confirmed in The Jackson Laboratory colony.
A targeting vector was designed to replace exon 1 of the nitric oxide synthase 1, neuronal (Nos1) gene with a neomycin cassette. This exon encodes the initiation site and amino acids 1 - 159 of the protein. The targeting vector was electroporated into 129S4/SvJae -derived Jl ES cells that were injected into C57BL/6 blastocysts. Injected blastocysts were implanted in (C57BL/6 x DBA/2)F1 mice. Chimeric progeny were backcrossed to C57BL/6 mice. Some mice were further backcrossed to C57BL/6J mice (Stock No. 000664) for more than 10 generations to produce Stock No. 002986.
Allele Name | targeted mutation 1, Paul L Huang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alphaNOS1; Kn; N2KO; nNOS-; nNOS KO; nNOS-; NOSdelta |
Gene Symbol and Name | Nos1, nitric oxide synthase 1, neuronal |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 5 |
Molecular Note | A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. |
Mutations Made By | Dr. Paul Huang, Mass General Hospital, Harvard Med Sch |
This strain may be maintained by breeding heterozygous siblings due to some lethality related to stomach hypertrophy observed in homozygotes.
When using the nNos KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002633 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Nos1<tm1Plh> |
Frozen Mouse Embryo | B6;129S4-Nos1<tm1Plh>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Nos1<tm1Plh>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Nos1<tm1Plh>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Nos1<tm1Plh>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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