B6;129S4-Nos1^tm1Plh/J

Stock number: 002633
Product name: nNos KO
Research areas: Diabetes and Obesity Research, Neurobiology Research

Description

Nos1 KO mice may useful when studying structure and function of neurons and their synaptic connections in the brain and periphery.

Detailed description

The nitric oxide signaling molecule produced by the neuronal Nos1 gene is widely expressed, affecting diverse systems. The knockout of this gene has been shown to affect such normal characteristics as cognition and behavior, long-term memory, cortical development, colonic motility, skeletal muscle contraction, ovarian cycle regulation, cardiac/cardiovascular function, and even sensitivity to Anthrax lethal toxin. Heterozygous mice are viable and fertile, while some lethality is seen in homozygotes due to stomach hypertrophy. Homozygotes have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer (Huang et al., 1993). Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. Homozygous males are reported to be more aggressive than normal wildtype siblings although this has not been confirmed in The Jackson Laboratory colony.

Development

A targeting vector was designed to replace exon 1 of the nitric oxide synthase 1, neuronal (Nos1) gene with a neomycin cassette. This exon encodes the initiation site and amino acids 1 - 159 of the protein. The targeting vector was electroporated into 129S4/SvJae -derived Jl ES cells that were injected into C57BL/6 blastocysts. Injected blastocysts were implanted in (C57BL/6 x DBA/2)F1 mice. Chimeric progeny were backcrossed to C57BL/6 mice. Some mice were further backcrossed to C57BL/6J mice (Stock No. 000664) for more than 10 generations to produce Stock No. 002986.

Allele

Symbol: Nos1^tm1Plh
Name: targeted mutation 1, Paul L Huang
MGI accession ID: MGI:1857227
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Nos1
Marker name: nitric oxide synthase 1, neuronal
Chromosome: 5
Strain of origin: 129S4/SvJae
Molecular note: A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain.