B6.C3-Lmx1a^dr-6J/J

Stock number: 002624
Product name: dreher 6 Jackson
Research areas: Dermatology Research, Neurobiology Research, Reproductive Biology Research, Sensorineural Research

Detailed description

These mice carry a spontaneous re-mutation of the mouse dreher (Lmx1a^dr) gene that causes skeletal defects, hyperactivity, abnormal gait, deafness, white belly spotting, and hypoplasia of Mullerian duct derivatives.

Development

The dreher 6 Jackson mutation arose spontaneously at The Jackson Laboratory in 1992 in the strain C3H/HeJ-Pou1f1^dw-J/J (stock#000510). After 9 generations of intercrossing on this background, a C3H/HeSnJ male was bred with an ovarian transplant host from a dr-6J/dr-6J donor and their offspring were intercrossed. No dwarf mice had been seen in many years of breeding indicating the dwarf Jackson mutation had been bred out. At generation +F9N1F7 a heterozygote, identified through C3H derived sequence at D1Mit425, D1Mit202, and D1Mit 15, was outcrossed to C57BL/6JEi and the new strain was maintained by backcrossing MIT marker-typed heterozygotes to C57BL/6JEi. Between 2000 and 2002, embryos were cryopreserved from C57BL/6J females bred with heterozygous males at or beyond generation N5.

Allele

Symbol: Lmx1a^dr-6J
Name: dreher 6 Jackson
MGI accession ID: MGI:1856498
Generation method: Spontaneous
Marker symbol: Lmx1a
Marker name: LIM homeobox transcription factor 1 alpha
Marker synonyms: DFNA7; LMX1; Lmx1.1; shaker short-tail
Chromosome: 1
Strain of origin: C3H/HeJ-Pou1f1^dw-J/J
Molecular note: The deletion of exons 4-8 prevented protein production.