Histological assessment of corneas from mice homozygous for the Dstn corn1-2J mutation reveals focal epithelial thickening and enlarged surface epithelial cells with degenerating nuclei. These mice lack the neovascularization found in mice homozygous for the related Dstn corn1 mutation (Stock No. 001649) and have a more mild corneal epithelial phenotype. Corneal epithelial cells show increased levels and altered organizational patterns of filamentous actin.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Dstn | destrin |
Histological assessment of corneas from mice homozygous for the Dstn corn1-2J mutation reveals focal epithelial thickening and enlarged surface epithelial cells with degenerating nuclei. These mice lack the neovascularization found in mice homozygous for the related Dstn corn1 mutation (Stock No. 001649) and have a more mild corneal epithelial phenotype. Preliminary assessment indicates that this variation in phenotype results from the distinct mutations in Dstn, not from differences in genetic background. In both mutants the corneal epithelial cells show increased levels and altered organizational patterns of filamentous actin. In the Dstncorn1 mutants this alteration to filamentous actin has been shown to result in accelerated proliferation of basal corneal epithelial cells.
The corn1-2J allele of Dstn was identified in the C57BL/6JSmn inbred strain at The Jackson Laboratory in approximately 1991. In 2012 sperm was cryopreserved from homozygous males.
Allele Name | corneal disease 1, 2 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Dstn, destrin |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 2 |
Molecular Note | The corn1-2J mouse was identified as an allele of corn1 by complementation analysis. The mutation was identifed as a C-to-T transition at coding nucleotide position 316 in exon 3. This mutation results in a Pro106Ser amino acid change that is predicted to affect the binding of the protein to F- and G-actin. |
When using the C57BL/6JSmn-Dstncorn1-2J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Dstn<corn1-2J> |
Frozen Mouse Embryo | C57BL/6JSmn-Dstn<corn1-2J>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6JSmn-Dstn<corn1-2J>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6JSmn-Dstn<corn1-2J>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6JSmn-Dstn<corn1-2J>/J | $3373.50 |
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