These tetO-βgal mice express a transgene containing beta-galactosidase (lacZ) reporter gene under the control of a tetracycline-responsive promoter element (TRE; tetO).
Dr. Lothar Hennighausen, National Institutes of Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible) |
B6;SJL-Tg(tetoplacZ)2Mam transgenic mice serve as reporter/tester mice for transgenic mice expressing the tetracycline-controlled transactivator proteins tTA or rtTA. This strain carries a beta-galactosidase (lacZ) reporter gene under the control of a tetracycline-responsive promoter element (TRE; tetO), consisting of the human cytomegalovirus early promoter fused to tet operator sequences. When these mice are mated with transgenic mice expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawl, if tTA is used) of tetracycline, or its analog, doxycyline. Beta-galactosidase activity is not observed in the absence of tTA or rtTA transgene.
A transgenic construct containing a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal was injected into fertilized B6;SJL mouse eggs. Founder animals were obtained.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | When crossed with a strain expressing rTA or rtTA, expression of the transgene may be induced in the bitransgenic progeny by the administration or withdrawal of tetracycline or its analog, doxycycline. |
Allele Name | transgene insertion 2, Lothar Hennighausen |
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Allele Type | Transgenic (Reporter, Inducible) |
Allele Synonym(s) | TetO-beta-gal; tTAluc |
Gene Symbol and Name | Tg(tetop-lacZ)2Mam, transgene insertion 2, Lothar Hennighausen |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | When crossed with a strain expressing rTA or rtTA, expression of the transgene may be induced in the bitransgenic progeny by the administration or withdrawal of tetracycline or its analog, doxycycline. |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
General Note | Four lines were produced (G1-G4). In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene. |
Molecular Note | The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. |
While maintaining a live colony, these mice are bred as hemizygotes.
When using the tetO-βgal mouse strain in a publication, please cite the originating article(s) and include JAX stock #002621 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetop-lacZ)2Mam |
Frozen Mouse Embryo | B6;SJL-Tg(tetop-lacZ)2Mam/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(tetop-lacZ)2Mam/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(tetop-lacZ)2Mam/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(tetop-lacZ)2Mam/J Frozen Embryo | $3373.50 |
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