B6;SJL-Tg(tetop-lacZ)2Mam/J

Stock number: 002621
Product name: tetO-βgal
Research areas: Research Tools

Description

These tetO-βgal mice express a transgene containing beta-galactosidase (lacZ) reporter gene under the control of a tetracycline-responsive promoter element (TRE; tetO).

Detailed description

B6;SJL-Tg(tetoplacZ)2Mam transgenic mice serve as reporter/tester mice for transgenic mice expressing the tetracycline-controlled transactivator proteins tTA or rtTA. This strain carries a beta-galactosidase (lacZ) reporter gene under the control of a tetracycline-responsive promoter element (TRE; tetO), consisting of the human cytomegalovirus early promoter fused to tet operator sequences. When these mice are mated with transgenic mice expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawl, if tTA is used) of tetracycline, or its analog, doxycyline. Beta-galactosidase activity is not observed in the absence of tTA or rtTA transgene.

Development

A transgenic construct containing a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal was injected into fertilized B6;SJL mouse eggs. Founder animals were obtained.

Allele

Symbol: Tg(tetop-lacZ)2Mam
Name: transgene insertion 2, Lothar Hennighausen
MGI accession ID: MGI:3053973
Generation method: Transgenic
Attributes: Reporter; Inducible
Synonyms: TetO-beta-gal; tTAluc
Marker symbol: Tg(tetop-lacZ)2Mam
Marker name: transgene insertion 2, Lothar Hennighausen
Marker synonyms: TetO-beta-gal
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: When crossed with a strain expressing rTA or rtTA, expression of the transgene may be induced in the bitransgenic progeny by the administration or withdrawal of tetracycline or its analog, doxycycline.
Molecular note: The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene.
General note: Four lines were produced (G1-G4).

In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene.