This consomic is valuable in the study of sex determination. The TOrl /+ XYRF offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YRF/J males, are sex reversed, having ovarian tissue development.
The Y Chromosome was backcrossed from RF/J onto C57BL/6JEi through repeated backcrossing. In 1996 embryos were cryopreserved from C57BL/6JEi females bred with consomic males at generation N22 or beyond.
Currently there are no related genes or alleles for this strain.