STOCK Tg(TcrHEL3A9)1Mmd/J

Stock number: 002597
Product name: 3A9 TCR
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

Mice carrying the (TcrHEL3A9) transgene express the alpha and beta chains of a T-cell receptor specific for hen egg lysozyme. Transgenics are used to study maintenance and breakdown of immunological tolerance, overcoming of the anergic state, and issues of costimuli.

Allele

Symbol: Tg(TcrHEL3A9)1Mmd
Name: transgene insertion 1, Mark M Davis
MGI accession ID: MGI:2385683
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcrHEL3A9)1Mmd
Marker name: transgene insertion 1, Mark M Davis
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Transgenic mice produced from this construct carry transgenes encoding the T cell receptor alpha and beta (Tcra and Tcrb, respectively) chains from the hen egg lysozyme (HEL)-specific T cell hybridoma 3A9. Two vectors were constructed and subsequently co-injected into C57BL/6J eggs. The first vector contained the Tcra variable and joining regions (V and J, respectively) and a 3.5kb segment 3' to the Tcra constant region. The second vector contained the Tcrb variable, diversity, and joining regions (V, D, and J, respectively) and a 7.5kb segment 3' to the Tcrb constant region, which included the Tcrb enhancer region. Transgenic mice expressed the alpha and beta chains of a T cell receptor specific for hen egg lysozyme. Two female founders were generated.