Mice homozygous for the Ifngtm1Ts targeted mutation are viable and fertile. This Ifng -deficient mutant develops type 1 diabetes at the same rate as the NOD/ShiLt parental strain. Specific pathogen free (SPF) conditions are critical as these mice have diminished immune responses to pathogens.
The mutation originally generated on a B6,129 background by Dr. Timothy Stewart (strain 002287) was congenically transferred to the NOD/Lt background using a marker assisted protocol and fixed to homozygosity at backcross generation N7 by Dr. David Serreze, The Jackson Laboratory, Bar Harbor. A genome scan confirmed the stock to be homozygous for linkage markers delineating twenty-three previously identified, insulin dependent diabetes susceptibility loci of NOD/Lt origin.
|Allele Name||targeted mutation 1, Timothy Stewart|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||gko; ifgtm1; Ifntm/Ts; ifntm1Ts; IFN-g KO; ifng-; Ifngtm1Ts; Ifn-gtm1Ts; IFN-gamma-; IFNgamma-; IFNgamma KO; IFN-gamma KO; IFN-gammatm1Ts; IFN-gammaKO; Ifngtm1Ts|
|Gene Symbol and Name||Ifng, interferon gamma|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.|
|Mutations Made By|| |
Dr. Timothy Stewart
When maintaining a live colony, homozygous mice may be bred together. Specific pathogen free (SPF) conditions are critical as these mice have diminished immune responses to pathogens.
When using the NOD.IFNnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #002575 in your Materials and Methods section.