NOD.129S7(B6)-Ifng^tm1Ts/DvsJ

Stock number: 002575
Product name: NOD.IFN^null
Research areas: Cancer Research, Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These Ifng knock-out mice develop type 1 diabetes.

Detailed description

Mice homozygous for the Ifng^tm1Ts targeted mutation are viable and fertile. This Ifng -deficient mutant develops type 1 diabetes at the same rate as the NOD/ShiLt parental strain. Specific pathogen free (SPF) conditions are critical as these mice have diminished immune responses to pathogens.

Development

The mutation originally generated on a B6,129 background by Dr. Timothy Stewart (strain 002287) was congenically transferred to the NOD/Lt background using a marker assisted protocol and fixed to homozygosity at backcross generation N7 by Dr. David Serreze, The Jackson Laboratory, Bar Harbor. A genome scan confirmed the stock to be homozygous for linkage markers delineating twenty-three previously identified, insulin dependent diabetes susceptibility loci of NOD/Lt origin.

Allele

Symbol: Ifng^tm1Ts
Name: targeted mutation 1, Timothy Stewart
MGI accession ID: MGI:1857184
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifng
Marker name: interferon gamma
Chromosome: 10
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.