Heterozygotes and homozygotes have accelerated diabetes onset, compared with NOD controls, with homozygotes dying by 5 to 7 weeks of age and heterozygotes having an average lifespan of approximately 100 days. Treatment with complete Freund?s adjuvant can delay disease onset and extend lifespan to permit homozygotes to reproduce.
The Il2tm1Hor targeted mutation was backcrossed from B6.129P2-Il2tm1Hor/J onto NOD/ShiLtDvs in the laboratory of Dr. David Serreze at The Jackson Laboratory. In 2006 and 2007 embryos were generated for cryopreservation from males that were heterozygous and females that were either heterozygous or homozygous. The generation of the parents at the initiation of the freeze was N9F30.
|Allele Name||targeted mutation 1, Ivan Horak|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-2-; Il2-; IL-2 KO; IL-2null|
|Gene Symbol and Name||Il2, interleukin 2|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Insertion of a neomycin-resistance gene into the third exon introduced several stop codons in all reading frames.|
|Mutations Made By|| |
Dr. Ivan Horak, Forschungs institut fur Molekulare
Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the Il-2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002573 in your Materials and Methods section.