NOD.Cg-Prkdc^scid B2m^tm1Unc/J

Stock number: 002570
Product name: NOD scid β2m^null
Research areas: Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Metabolism Research, Research Tools, Virology Research

Description

These mice are doubly homozygous for the severe combined immunodeficiency (scid) mutation and the beta2m null allele. They lack MHC class I, exhibit low NK cell activity, and support markedly elevated levels of human T cell engraftment.

Detailed description

Mice homozygous for both the B2m^tm1Unc and Prkdc^scid (commonly referred to as scid) mutations on the NOD/ShiLtSz background are class I deficient, B and T cell deficient, C-5 deficient (Hc⁰), and have low NK cells. This strain is an ideal model for xenograft transplantation studies and is an excellent source for insulitis-free, MHC class I-negative islets for transplantation studies.

Development

The B2m^tm1Unc targeted mutant strain was developed in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at the University of North Carolina at Chapel Hill. It was generated by a targeted disruption of the B2m gene. The 129-derived E14TG2a ES cell line was used. This strain carrying both B2m and scid mutations was generated in the laboratory of Dr. Leonard Shultz at the Jackson Laboratory by backcrossing the B2m^tm1Unc mutation 10 generations to the NOD/LtSz-Prkdc^scid strain.

Allele

Symbol: B2m^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857133
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: B2m
Marker name: beta-2 microglobulin
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of a neomycin-resistance gene into the second exon.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).