These mice are doubly homozygous for the severe combined immunodeficiency (scid) mutation and the beta2m null allele. They lack MHC class I, exhibit low NK cell activity, and support markedly elevated levels of human T cell engraftment.
Dr. Leonard D. Shultz, The Jackson Laboratory
Mice homozygous for both the B2mtm1Unc and Prkdcscid (commonly referred to as scid) mutations on the NOD/ShiLtSz background are class I deficient, B and T cell deficient, C-5 deficient (Hc0), and have low NK cells. This strain is an ideal model for xenograft transplantation studies and is an excellent source for insulitis-free, MHC class I-negative islets for transplantation studies.
The B2mtm1Unc targeted mutant strain was developed in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at the University of North Carolina at Chapel Hill. It was generated by a targeted disruption of the B2m gene. The 129-derived E14TG2a ES cell line was used. This strain carrying both B2m and scid mutations was generated in the laboratory of Dr. Leonard Shultz at the Jackson Laboratory by backcrossing the B2mtm1Unc mutation 10 generations to the NOD/LtSz-Prkdcscid strain.
|Allele Name||targeted mutation 1, University of North Carolina|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, University of North Carolina; B2mtm1Unc|
|Gene Symbol and Name||B2m, beta-2 microglobulin|
|Gene Synonym(s)||beta 2 microglobulin; beta2-m; Ly-m11; lymphocyte antigen m11; Ly-m11; IMD43|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Insertion of a neomycin-resistance gene into the second exon.|
|Mutations Made By|| |
Dr. Oliver Smithies, University of North Carolina at Chapel Hill
|Allele Name||severe combined immunodeficiency|
|Allele Synonym(s)||Prkdcscid; severe combined immunodeficiency|
|Gene Symbol and Name||Prkdc, protein kinase, DNA activated, catalytic polypeptide|
|Gene Synonym(s)||AI326420; DNA-PK; DNA-PKcs; DNAPDcs; DNPK1; DOXNPH; doxorubicin nephropathy; DNAPK; expressed sequence AU019811; dxnph; dxnph; scid; IMD26; HYRC; HYRC1; p350; slip; XRCC7; severe combined immunodeficiency; expressed sequence AI326420; AU019811; DNA-PKC; DNAPKc|
|Site of Expression||T and B lymphocytes.|
|Strain of Origin||C.BKa-Ighb/Icr|
|Molecular Note||A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon.|
This B2mtm1Unc strain is maintained by mating homozygous siblings. Only homozygous mice may be ordered. Reproduction is good. Expected coat color from breeding:Albino
When using the NOD scid β2mnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #002570 in your Materials and Methods section.
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