Mice homozygous for a targeted mutation in the Myf5 gene die perinatally from respiratory failure due to improper development of the rib cage. Ribs are truncated and the sternum is shortened, with no sternebral segmentation and no connection to the ribs. Mutant mice show no obvious alterations in the vertebral column in comparison to wild type controls, and there are no skeletal muscle abnormalities apparent at birth.
|Allele Name||targeted mutation 1, Rudolf Jaenisch|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Rudolf Jaenisch; Myf5tm1Jae|
|Gene Symbol and Name||Myf5, myogenic factor 5|
|Gene Synonym(s)||RIKEN cDNA B130010J22 gene; Myf-5; B130010J22Rik; bHLHc2; B130010J22Rik; EORVA|
|Strain of Origin||129S4/SvJae|
|General Note||Mice homozygous for Myf6 mutations lack or show reduced Myf5 expression and exhibit phenotypes seen in Myf5tm1Jae homozygotes. Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:24360, J:31636).|
|Molecular Note||A PGK-neomycin cassette was inserted into exon 1 of the gene, disrupting the reading frame.|
|Mutations Made By|| |
Dr. Rudolf Jaenisch, Whitehead Institute (MIT)
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to BALB/cJ inbred mice (Stock No. 000651). Mice homozygous for the mutation are not viable (perinatal lethal).
When using the C.129S4-Myf5tm1Jae/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002522 in your Materials and Methods section.
|Heterozygous or Wild-type for Myf5<tm1Jae>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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