These plasminogen activator inhibitor-1 deficient mice may be useful for studying the role of fibrosis and fibrinolysis in amyloid plaque deposition (Alzheimer's disease), hyperphagia, obesity, injury and muscular dystrophy.
Dr. Peter Carmeliet, University of Leuven
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Serpine1 | serine (or cysteine) peptidase inhibitor, clade E, member 1 |
Mice homozygous for this plasminogen activator inhibitor-1 null allele (PAI-1-) are viable and fertile with normal development. Compared to wildtype mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wildtype mice. Thus, disruption of the PAI-1 (Serpine1) gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.
The plasminogen activator inhibitor-1 (PAI-1 or Serpine1) targeted null mutation was made in the laboratories of Dr. Richard Mulligan (Whitehead Institute for Biomedical Sciences) and of Dr. Peter Carmellet (University of Leuven). A targeting vector was used which resulted in the neomycin-resistant gene replacing all coding sequences. The Serpine1 gene was isolated from a BALB/c genomic library and targeting was done in D3 ES cells.
Allele Name | targeted mutation 1, Richard C Mulligan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PAI-1-; Planh1tm1; Serpine1tm1Mg |
Gene Symbol and Name | Serpine1, serine (or cysteine) peptidase inhibitor, clade E, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 5 |
Molecular Note | A PGK-neomycin resistance cassette replaced all of the Serpine1 coding sequence and part of the promoter region including the transcription initiation site. Northern blot analysis did not detect Serpine1 mRNA in endotoxin-treated homozygous mutant liver. Neither untreated nor endotoxin-treated homozygous mutant kidney produced detectable SERPINE1 immunoreactivity. A monoclonal antibody based immunofunctional assay did not detect protein levels in plasma of endotoxin-treated homozygous mutant mice. |
Mutations Made By | Dr. Peter Carmeliet, University of Leuven |
When using the PAI-1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002507 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Serpine1<tm1Mlg> |
Frozen Mouse Embryo | B6.129S2-Serpine1<tm1Mlg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Serpine1<tm1Mlg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Serpine1<tm1Mlg>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Serpine1<tm1Mlg>/J Frozen Embryos | $3373.50 |
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