Mice carrying both the (CD3E)26Cpt transgene (Stock No. 002194) and the Rag1^tm1Mom targeted mutation exhibits characteristics of both mutant strains. Double homozygous mutant mice produce no mature T, B, or NK cells. They have no CD3⁺ or T cell receptor (TCR) alpha-beta positive cells. The thymus of the mutant mice contains 15 to 130 times fewer cells than heterozygous or wildtype siblings. The thymocytes are CD8^-CD4^- and most are IL2 receptor positive. Neither the spleen nor bone marrow contain any IgM or IgD staining cells, indicating an absence of mature B cells. These and other data suggest that B cell and T cell development has been arrested at an early stage.

Expression Data

Expressed Gene	CD3E, CD3e molecule, human
Site of Expression
Site of Expression	expression is seen in bone marrow derived cell lines.

Control Suggestions

This strain is a mixture of C57BL/6, CBA and 129 inbred strains. Thus, there are no appropriate physiological controls. If a control is necessary C57BL/6J (Stock No. 000664), CBA/J (Stock No. 000656), and 129P3/J (000690) may be used. These strains also serve as DNA control.
000656 CBA/J
000664 C57BL/6J
000690 129P3/J

Additional Information

Considerations for Choosing Controls

Selected References

Wang B; Biron C; She J; Higgins K; Sunshine MJ; Lacy E; Lonberg N; Terhorst C. 1994. A block in both early T lymphocyte and natural killer cell development in transgenic mice with high-copy numbers of the human CD3E gene. Proc Natl Acad Sci U S A 91(20):9402-6PubMed: 7937778 MGI: J:81711

View All References

Genetics

Tg(CD3E)26Cpt

Allele Symbol: Tg(CD3E)26Cpt

Allele Name	transgene insertion 26, Cox Terhorst
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	CD3epsilon; CD3epsilontg26; tgE26; Tgepsilon26
Gene Symbol and Name	Tg(CD3E)26Cpt, transgene insertion 26, Cox Terhorst
Gene Synonym(s)
Promoter	CD3E, CD3e molecule, human
Expressed Gene	CD3E, CD3e molecule, human
Strain of Origin	(C57BL/6J x CBA/J)F2
Chromosome	17
Molecular Note	The transgene contains a human gene encoding the CD3 antigen, epsilon polypeptide sequences. 30-35 copies of the transgene integrated into the genome between Sstr5 and Metrn on Chromosome 17, accompanied by duplication of the neighboring genomic region spanning 323 kb. Genes surrounding the integrated tg26 transgene are not disrupted.
Mutations Made By	Cox Terhorst, Beth Israel Deaconess Medical Center / Harvard Medical School

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Allele Name	targeted mutation 1, Peter Mombaerts
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Rag-; Rag^1tm1Mom; RAG-1-; Rag1^-; RAG1^null; Rag-1KO
Gene Symbol and Name	Rag1, recombination activating gene 1
Gene Synonym(s)
Site of Expression	expression is seen in bone marrow derived cell lines.
Strain of Origin	129S7/SvEvBrd-Hprt⁺
Chromosome	2
Molecular Note	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Mutations Made By	Peter Mombaerts, Max Planck Research Unit for Neurogenetics

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1^tm1Mom related

Immunology, Inflammation and Autoimmunity Research
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
- Immunodeficiency
  - B and T cell deficiency
- Inflammation
  - B and T cell deficiency
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
Hematological Research
- Immunological Defects
  - B and T cell deficiency

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B and T cell deficiency
  - B, T, and NK cell deficiency

Mammalian Phenotype Terms by Genotype

References

Wang B; Biron C; She J; Higgins K; Sunshine MJ; Lacy E; Lonberg N; Terhorst C. 1994. A block in both early T lymphocyte and natural killer cell development in transgenic mice with high-copy numbers of the human CD3E gene. Proc Natl Acad Sci U S A 91(20):9402-6PubMed: 7937778 MGI: J:81711

Additional References

Mombaerts P; Clarke AR; Rudnicki MA; Iacomini J; Itohara S; Lafaille JJ; Wang L; Ichikawa Y; Jaenisch R; Hooper ML; Tonegawa S. 1992. Mutations in T-cell antigen receptor genes alpha and beta block thymocyte development at different stages [published erratum appears in Nature 1992 Dec 3;360(6403):491] Nature 360(6401):225-31PubMed: 1359428 MGI: J:3206
Mombaerts P; Iacomini J; Johnson RS; Herrup K; Tonegawa S; Papaioannou VE. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68(5):869-77PubMed: 1547488 MGI: J:1934

Additional - Rag1^tm1Mom related

Additional - Tg(CD3E)26Cpt related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(CD3E)26Cpt
- Genotyping resources and troubleshooting
Citation
When using the STOCK Tg(CD3E)26Cpt-Rag1^tm1Mom/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002506 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Tg(CD3E)26Cpt, Homozygous for Rag1<tm1Mom>, 1 pair minimum

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description