These double mutant mice produce no mature T, B, or NK cells.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Mice carrying both the (CD3E)26Cpt transgene (Stock No. 002194) and the Rag1tm1Mom targeted mutation exhibits characteristics of both mutant strains. Double homozygous mutant mice produce no mature T, B, or NK cells. They have no CD3+ or T cell receptor (TCR) alpha-beta positive cells. The thymus of the mutant mice contains 15 to 130 times fewer cells than heterozygous or wildtype siblings. The thymocytes are CD8-CD4- and most are IL2 receptor positive. Neither the spleen nor bone marrow contain any IgM or IgD staining cells, indicating an absence of mature B cells. These and other data suggest that B cell and T cell development has been arrested at an early stage.
Expressed Gene | CD3E, CD3e molecule, human |
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Site of Expression |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion 26, Cox Terhorst |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | CD3epsilon; CD3epsilontg26; tgE26; Tgepsilon26 |
Gene Symbol and Name | Tg(CD3E)26Cpt, transgene insertion 26, Cox Terhorst |
Gene Synonym(s) | |
Promoter | CD3E, CD3e molecule, human |
Expressed Gene | CD3E, CD3e molecule, human |
Strain of Origin | (C57BL/6J x CBA/J)F2 |
Chromosome | 17 |
Molecular Note | The transgene contains a human gene encoding the CD3 antigen, epsilon polypeptide sequences. 30-35 copies of the transgene integrated into the genome between Sstr5 and Metrn on Chromosome 17, accompanied by duplication of the neighboring genomic region spanning 323 kb. Genes surrounding the integrated tg26 transgene are not disrupted. |
Mutations Made By | Cox Terhorst, Beth Israel Deaconess Medical Center / Harvard Medical School |
When using the STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002506 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Tg(CD3E)26Cpt, Homozygous for Rag1<tm1Mom>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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