STOCK Rag1^tm1Mom Tg(CD3E)26Cpt/J

Stock number: 002506
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

These double mutant mice produce no mature T, B, or NK cells.

Detailed description

Mice carrying both the (CD3E)26Cpt transgene (Stock No. 002194) and the Rag1^tm1Mom targeted mutation exhibits characteristics of both mutant strains. Double homozygous mutant mice produce no mature T, B, or NK cells. They have no CD3⁺ or T cell receptor (TCR) alpha-beta positive cells. The thymus of the mutant mice contains 15 to 130 times fewer cells than heterozygous or wildtype siblings. The thymocytes are CD8^-CD4^- and most are IL2 receptor positive. Neither the spleen nor bone marrow contain any IgM or IgD staining cells, indicating an absence of mature B cells. These and other data suggest that B cell and T cell development has been arrested at an early stage.

Allele

Symbol: Tg(CD3E)26Cpt
Name: transgene insertion 26, Cox Terhorst
MGI accession ID: MGI:3052629
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CD3E)26Cpt
Marker name: transgene insertion 26, Cox Terhorst
Chromosome: 17
Strain of origin: (C57BL/6J x CBA/J)F2
Expressed genes: CD3E,CD3e molecule,human
Molecular note: The transgene contains a human gene encoding the CD3 antigen, epsilon polypeptide sequences. 30-35 copies of the transgene integrated into the genome between Sstr5 and Metrn on Chromosome 17, accompanied by duplication of the neighboring genomic region spanning 323 kb. Genes surrounding the integrated tg26 transgene are not disrupted.

Allele

Symbol: Rag1^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857241
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rag1
Marker name: recombination activating gene 1
Chromosome: 2
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Site of expression: expression is seen in bone marrow derived cell lines.
Molecular note: A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.