B6.Cg-Tg(WapIGFBP3)67Dlr/J

Stock number: 002499

Description

These mice express a transgene containing the human insulin-like growth factor binding protein 3 gene.

Detailed description

Mice homozygous for the WapIGFBP3 transgene are viable and fertile. The transgene is expressed at high levels during lactation and post-lactation. There were no obvious changes in mammary gland histomorphology during lactation, but at 5 to 10 days post-laction, there was a delay in involution and decreased apoptosis. This phenotype is identical to that of the C57BL/6J-TgN(WapIgf1)39Dlr strain mice.

Development

The transgenic construct consists of the mouse whey acidic protein promoter followed by the complete open reading frame of the human insulin-like growth factor binding protein 3 gene. The construct was injected into the pronuclei of (C57BL/6 x SJL)F2 zygotes. Founder mice were crossed to C57BL/6 mice for a minimum of 5 generations.

Allele

Symbol: Tg(WapIGFBP3)67Dlr
Name: transgene insertion 67, Derek LeRoith
MGI accession ID: MGI:3053842
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(WapIGFBP3)67Dlr
Marker name: transgene insertion 67, Derek LeRoith
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: IGFBP3,insulin like growth factor binding protein 3,human
Molecular note: The transgene contains the mouse whey acidic protein promoter followed by the complete open reading frame of the human insulin-like growth factor binding protein 3 gene, including 116 bp of 5'UTR sequence and 523 bp of 3'UTR sequence.