These mice express a transgene containing the human insulin-like growth factor binding protein 3 gene.
Dr. Derek LeRoith, Mount Sinai School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice homozygous for the WapIGFBP3 transgene are viable and fertile. The transgene is expressed at high levels during lactation and post-lactation. There were no obvious changes in mammary gland histomorphology during lactation, but at 5 to 10 days post-laction, there was a delay in involution and decreased apoptosis. This phenotype is identical to that of the C57BL/6J-TgN(WapIgf1)39Dlr strain mice.
The transgenic construct consists of the mouse whey acidic protein promoter followed by the complete open reading frame of the human insulin-like growth factor binding protein 3 gene. The construct was injected into the pronuclei of (C57BL/6 x SJL)F2 zygotes. Founder mice were crossed to C57BL/6 mice for a minimum of 5 generations.
Expressed Gene | IGFBP3, insulin like growth factor binding protein 3, human |
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Site of Expression |
Allele Name | transgene insertion 67, Derek LeRoith |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(WapIGFBP3)67Dlr, transgene insertion 67, Derek LeRoith |
Gene Synonym(s) | |
Promoter | Wap, whey acidic protein, rat |
Expressed Gene | IGFBP3, insulin like growth factor binding protein 3, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The transgene contains the mouse whey acidic protein promoter followed by the complete open reading frame of the human insulin-like growth factor binding protein 3 gene, including 116 bp of 5'UTR sequence and 523 bp of 3'UTR sequence. |
Mutations Made By | Dr. Derek LeRoith, Mount Sinai School of Medicine |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygous viability/fertility has not been tested
When using the B6.Cg-Tg(WapIGFBP3)67Dlr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002499 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(WapIGFBP3)67Dlr |
Frozen Mouse Embryo | B6.Cg-Tg(WapIGFBP3)67Dlr/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(WapIGFBP3)67Dlr/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(WapIGFBP3)67Dlr/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(WapIGFBP3)67Dlr/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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