Mice homozygous for Il4tm2Nnt have significantly reduced levels of IgGl and IgE and reduced ability to produce Th2-derived cytokines. Mice are susceptible to infection by Leishmania major.Read More +
Mice homozygous for the Il4tm2Nnt targeted mutation are viable and fertile. T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced. Susceptible to infection by Leishmania major despite deficiency in IL4.
This strain was developed by Dr. Nancy Noben-Trauth and Dr. Birgit Ledermann at Sandoz Pharma Ltd. in Basel, Switzerland. A BALB/c-derived ES cell line was used.
|Allele Name||targeted mutation 2, Nancy Noben-Trauth|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Il4, interleukin 4|
|Strain of Origin||BALB/cJ|
|General Note||Susceptibility to Leishmania major in BALB/c mice has been ablated by introduction of a null mutant of the Il4 gene (J:35236), but in that study the introduced mutant came from a strain 129 background, and 129 is resistant to L. major infection. In mice carrying one of Noben-Trauth's null mutants, targeted in BALB/c embryonic stem cells, the loss of Il4 expression did not remove susceptibility to infection (J:31486).|
|Molecular Note||A neomycin resistance cassette was inserted into exon 3 of the gene.|
|Mutations Made By|| |
The strain is maintained by homozygous sibling matings. Expected coat color from breeding:Albino
When using the IL-4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002496 in your Materials and Methods section.
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