CBA/CaGnLe-Cryaa^lop18/J

Stock number: 002489
Product name: lens opacity 18
Research areas: Sensorineural Research

Description

These mice carry a spontaneous mutation at the Cryaa locus characterized by development of cataracts.

Detailed description

Mice homozygous for Cryaa^lop18 develop large, dense nuclear cataracts. At embryonic day (E) 10 the lens vesicle in these homozygotes buds out from the surface ectoderm. At E14 tiny vacuoles develop in the lens, these are larger at E16, and by four months of age there is advanced degeneration of the cortex with posterior migration of the lens epithelial nuclei and the posterior pole of the lens shows abnormal lens fibers. However, the cataracts were not found to progress further after this age. (Chang et al., 1996.)

Development

The recessive Cryaa^lop18 mutation arose spontaneously and was identified in the inbred strain CBA/CaGnLe at The Jackson Laboratory in approximately 1993. In 1995 embryos from homozygous parents were cryopreserved. The CBA/CaGnLeJ strain, Stock No. 001143, does not carry this mutation.

Allele

Symbol: Cryaa^lop18
Name: lens opacity 18
MGI accession ID: MGI:1858023
Generation method: Spontaneous
Marker symbol: Cryaa
Marker name: crystallin, alpha A
Chromosome: 17
Strain of origin: CBA/CaGnLeJ
Molecular note: A G-to-A transition at coding nucleotide 161 of the mRNA that converts codon 54 from an arginine to a histidine (p.R54H).