Mice homozygous for Cryaalop18 develop large, dense nuclear cataracts. At embryonic day (E) 10 the lens vesicle in these homozygotes buds out from the surface ectoderm. At E14 tiny vacuoles develop in the lens, these are larger at E16, and by four months of age there is advanced degeneration of the cortex with posterior migration of the lens epithelial nuclei and the posterior pole of the lens shows abnormal lens fibers. However, the cataracts were not found to progress further after this age. (Chang et al., 1996.)
The recessive Cryaalop18 mutation arose spontaneously and was identified in the inbred strain CBA/CaGnLe at The Jackson Laboratory in approximately 1993. In 1995 embryos from homozygous parents were cryopreserved. The CBA/CaGnLeJ strain, Stock No. 001143, does not carry this mutation.
|Allele Name||lens opacity 18|
|Gene Symbol and Name||Cryaa, crystallin, alpha A|
|Strain of Origin||CBA/CaGnLeJ|
|Molecular Note||A G-to-A transition at coding nucleotide 161 of the mRNA that converts codon 54 from an arginine to a histidine (p.R54H).|
When using the lens opacity 18 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002489 in your Materials and Methods section.