These Alox5 knock-out mice exhibit a selective opposition to certain inflammatory insults. They are suitable for use in applications related to the study of biological mediators of inflammation and anaphylaxis.Read More +
Mice homozygous for the Alox5tm1Fun targeted mutation are viable and fertile. In general, homozygous mutant mice are selectively resistant to inflammatory insults. They are resistant to the lethal effects of platelet activating factor but reaction to endotoxin shock is normal. Phorbol ester induced inflammation is normal but arachidonic acid induced inflammation is reduced. Commonly referred to as 5-LO.
This strain was developed in the lab of Dr. Colin Funk at Vanderbilt University School of Medicine.
|Allele Name||targeted mutation 1, Colin D Funk|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||5-LO KO; 5-LO-; 5LO-; 5-LOX K/O; 5-LOX-; 5LX-|
|Gene Symbol and Name||Alox5, arachidonate 5-lipoxygenase|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Insertion of a neomycin resistance cassette into exon 6 disrupted the Alox5 gene. RT-PCR studies did not detect transcript in resident peritoneal macrophages of homozygous mutant mice. Western blot analysis of resident peritoneal macrophages of homozygous mutant mice did not detect ALOX5.|
|Mutations Made By|| |
Colin Funk, Queen's University
Homozygous mice are viable and fertile. This strain is maintained by homozygous matings. Expected coat color from breeding:White Bellied Agouti
When using the 5LX- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002485 in your Materials and Methods section.