Overview

Also Known As: trkC^TK
Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Donating Investigator

Dr. Mariano Barbacid, Centro Nacional de Investigaciones Oncol

Genetic overview

Genetic Background	Generation

Ntrk3^tm1Bbd

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ntrk3	neurotrophic tyrosine kinase, receptor, type 3

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Research Applications

Neurobiology Research
Sensorineural Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Development

Part of exon K2 of the Ntrk3 gene was replaced using a vector containing the neo resistance gene. This strain was generated on a 129 genetic background. It has subsequently been bred to C57BL/6 mice.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Klein R; Silos-Santiago I; Smeyne RJ; Lira SA; Brambilla R; Bryant S; Zhang L; Snider WD; Barbacid M. 1994. Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements [see comments] Nature 368(6468):249-51PubMed: 8145824 MGI: J:17195

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Genetics

Ntrk3^tm1Bbd

Allele Symbol: Ntrk3^tm1Bbd

Allele Name	targeted mutation 1, Mariano Barbacid
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Ntrk3^tm1Bbd; targeted mutation 1, Mariano Barbacid
Gene Symbol and Name	Ntrk3, neurotrophic tyrosine kinase, receptor, type 3
Gene Synonym(s)	AW125844; TRKC; expressed sequence AW125844; gp145(trkC); GP145-TrkC; TrkC; trkC
Strain of Origin	129S2/SvPas
Chromosome	7
Molecular Note	Insertion of a neomycin cassette into exon K2, which encodes part of the kinase domain of the protein. An alternatively spliced mRNA was detectable in an RNase protection analysis on tissues derived from homozygous mutant mice; these transcripts spliced from exon K1 to K3. A partial protein product is predicted to be expressed from this allele.
Mutations Made By	Dr. Mariano Barbacid, Centro Nacional de Investigaciones Oncol

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ntrk3^tm1Bbd related

Neurobiology Research
- Hearing Defects
- Neurotrophic Factor Defects
- Receptor Defects
Sensorineural Research
- Hearing Defects
Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ntrk3^tm1Bbd/Ntrk3^tm1Bbd
involves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

athetotic walking movements
- by P10, homozygotes display athetotic walking movements

impaired coordination
- on a rotating dowel, homozygotes fail to maintain an upright posture and tumble off

impaired limb coordination
- even at rest, the limbs are held in abnormal positions in relation to the trunk

limb grasping
- when hung by the tail, homozygotes draw their limbs in towards their bodies

mammalian phenotype

behavior/neurological phenotype
- Normal - homozygotes respond normally to painful stimuli in their whisker pads as well as to pinching of their tail and foot

mortality/aging

postnatal lethality
- most homozygotes die by P21

premature death
- a few homozygotes live up to 4 months or longer

nervous system phenotype

abnormal cochlear IHC afferent innervation pattern
- at P1, IHCs are devoid of innervation
- developmental analysis indicates that, at E16.5, some cochlear nerve fibers initially reach their peripheral targets but subsequently fail to maintain innervation and degenerate
- in contrast, the innervation pattern of OHCs appears normal

abnormal lumbar dorsal root ganglion morphology
- newborn homozygotes show a 19% neuron loss in the lumbar dorsal root ganglia

abnormal sensory neuron innervation pattern
- homozygotes lack Ia muscle afferent projections to spinal motor neurons and have fewer large myelinated axons in the dorsal root and posterior columns of the spinal cord

small cochlear ganglion
- at P1, a 51% reduction in cochlear ganglion neuron number is observed
- large-sized type I neurons (innervating IHCs) are preferentially lost (62% reduction), whereas small-sized type II neurons (innervating OHCs) remain unaffected

small vestibular ganglion
- by P1, a 16% reduction in vestibular ganglion neuron number is observed
- Normal - however, both vestibular ganglia and vestibular sensory epithelia are histologically normal and their innervation patterns appear unaffected

growth/size/body region phenotype

decreased body size
- although normal at birth, homozygotes exhibit reduced body size at P4

hearing/vestibular/ear phenotype

abnormal cochlear IHC afferent innervation pattern
- at P1, IHCs are devoid of innervation
- developmental analysis indicates that, at E16.5, some cochlear nerve fibers initially reach their peripheral targets but subsequently fail to maintain innervation and degenerate
- in contrast, the innervation pattern of OHCs appears normal

abnormal cochlear sensory epithelium morphology
- at P1, the cochlear sensory epithelium appears thinner and less stratified than normal

References

Klein R; Silos-Santiago I; Smeyne RJ; Lira SA; Brambilla R; Bryant S; Zhang L; Snider WD; Barbacid M. 1994. Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements [see comments] Nature 368(6468):249-51PubMed: 8145824 MGI: J:17195

Additional References

Schimmang T; Minichiello L; Vazquez E; San Jose I; Giraldez F; Klein R; Represa J. 1995. Developing inner ear sensory neurons require TrkB and TrkC receptors for innervation of their peripheral targets. Development 121(10):3381-91PubMed: 7588071 MGI: J:29245
Minichiello L; Piehl F; Vazquez E; Schimmang T; Hokfelt T; Represa J; Klein R. 1995. Differential effects of combined trk receptor mutations on dorsal root ganglion and inner ear sensory neurons. Development 121(12):4067-75PubMed: 8575307 MGI: J:30344
White FA; Silos-Santiago I; Molliver DC; Nishimura M; Phillips H; Barbacid M; Snider WD. 1996. Synchronous onset of NGF and TrkA survival dependence in developing dorsal root ganglia. J Neurosci 16(15):4662-72PubMed: 8764654 MGI: J:34321
Fagan AM; Zhang H; Landis S; Smeyne RJ; Silos-Santiago I; Barbacid M. 1996. TrkA, but not TrkC, receptors are essential for survival of sympathetic neurons in vivo. J Neurosci 16(19):6208-18PubMed: 8815902 MGI: J:35563

Additional - Ntrk3^tm1Bbd related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Ntrk3^tm1Bbd
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, this mutant can be maintained by mating heterozygous siblings or by mating heterozygous mice with normal wildtype siblings. Homozygous mice die by 2-3 weeks of age.
- Additional Breeding and Husbandry Support
Citation
When using the trkC^TKcite the originating article(s) and include JAX stock #002481 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Ntrk3<tm1Bbd>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: trkCTK Mice homozygous for this Ntrk3tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ntrk3tm1Bbd

Allele Symbol: Ntrk3tm1Bbd

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ntrk3tm1Bbd related

Mammalian Phenotype Terms by Genotype

Genotype: Ntrk3tm1Bbd/Ntrk3tm1Bbdinvolves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

mammalian phenotype

mortality/aging

nervous system phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

References

Additional References

Additional - Ntrk3tm1Bbd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: trkC^TK
Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Ntrk3^tm1Bbd

Allele Symbol: Ntrk3^tm1Bbd

Genotype: Ntrk3^tm1Bbd related

Genotype: Ntrk3^tm1Bbd/Ntrk3^tm1Bbd
involves: 129S2/SvPas * C57BL/6

Additional - Ntrk3^tm1Bbd related