Overview

Also Known As: trkC^TK
Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Donating Investigator

Dr. Mariano Barbacid, Centro Nacional de Investigaciones Oncol

Genetic overview

Genetic Background	Generation

Ntrk3^tm1Bbd

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ntrk3	neurotrophic tyrosine kinase, receptor, type 3

View Genetics

Research Applications

Neurobiology Research
Sensorineural Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Development

Part of exon K2 of the Ntrk3 gene was replaced using a vector containing the neo resistance gene. This strain was generated on a 129 genetic background. It has subsequently been bred to C57BL/6 mice.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Klein R; Silos-Santiago I; Smeyne RJ; Lira SA; Brambilla R; Bryant S; Zhang L; Snider WD; Barbacid M. 1994. Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements [see comments] Nature 368(6468):249-51PubMed: 8145824 MGI: J:17195

View All References

Genetics

Ntrk3^tm1Bbd

Allele Symbol: Ntrk3^tm1Bbd

Allele Name	targeted mutation 1, Mariano Barbacid
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Ntrk3^tm1Bbd; targeted mutation 1, Mariano Barbacid
Gene Symbol and Name	Ntrk3, neurotrophic tyrosine kinase, receptor, type 3
Gene Synonym(s)	AW125844; TRKC; expressed sequence AW125844; gp145(trkC); GP145-TrkC; TrkC; trkC
Strain of Origin	129S2/SvPas
Chromosome	7
Molecular Note	Insertion of a neomycin cassette into exon K2, which encodes part of the kinase domain of the protein. An alternatively spliced mRNA was detectable in an RNase protection analysis on tissues derived from homozygous mutant mice; these transcripts spliced from exon K1 to K3. A partial protein product is predicted to be expressed from this allele.
Mutations Made By	Dr. Mariano Barbacid, Centro Nacional de Investigaciones Oncol

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ntrk3^tm1Bbd related

Neurobiology Research
- Hearing Defects
- Neurotrophic Factor Defects
- Receptor Defects
Sensorineural Research
- Hearing Defects
Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ntrk3^tm1Bbd/Ntrk3^tm1Bbd
involves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

athetotic walking movements
- by P10, homozygotes display athetotic walking movements

impaired coordination
- on a rotating dowel, homozygotes fail to maintain an upright posture and tumble off

impaired limb coordination
- even at rest, the limbs are held in abnormal positions in relation to the trunk

limb grasping
- when hung by the tail, homozygotes draw their limbs in towards their bodies

mammalian phenotype

behavior/neurological phenotype
- Normal - homozygotes respond normally to painful stimuli in their whisker pads as well as to pinching of their tail and foot

mortality/aging

postnatal lethality
- most homozygotes die by P21

premature death
- a few homozygotes live up to 4 months or longer

nervous system phenotype

abnormal cochlear IHC afferent innervation pattern
- at P1, IHCs are devoid of innervation
- developmental analysis indicates that, at E16.5, some cochlear nerve fibers initially reach their peripheral targets but subsequently fail to maintain innervation and degenerate
- in contrast, the innervation pattern of OHCs appears normal

abnormal lumbar dorsal root ganglion morphology
- newborn homozygotes show a 19% neuron loss in the lumbar dorsal root ganglia

abnormal sensory neuron innervation pattern
- homozygotes lack Ia muscle afferent projections to spinal motor neurons and have fewer large myelinated axons in the dorsal root and posterior columns of the spinal cord

small cochlear ganglion
- at P1, a 51% reduction in cochlear ganglion neuron number is observed
- large-sized type I neurons (innervating IHCs) are preferentially lost (62% reduction), whereas small-sized type II neurons (innervating OHCs) remain unaffected

small vestibular ganglion
- by P1, a 16% reduction in vestibular ganglion neuron number is observed
- Normal - however, both vestibular ganglia and vestibular sensory epithelia are histologically normal and their innervation patterns appear unaffected

growth/size/body region phenotype

decreased body size
- although normal at birth, homozygotes exhibit reduced body size at P4

hearing/vestibular/ear phenotype

abnormal cochlear IHC afferent innervation pattern
- at P1, IHCs are devoid of innervation
- developmental analysis indicates that, at E16.5, some cochlear nerve fibers initially reach their peripheral targets but subsequently fail to maintain innervation and degenerate
- in contrast, the innervation pattern of OHCs appears normal

abnormal cochlear sensory epithelium morphology
- at P1, the cochlear sensory epithelium appears thinner and less stratified than normal

References

Klein R; Silos-Santiago I; Smeyne RJ; Lira SA; Brambilla R; Bryant S; Zhang L; Snider WD; Barbacid M. 1994. Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements [see comments] Nature 368(6468):249-51PubMed: 8145824 MGI: J:17195

Additional References

Schimmang T; Minichiello L; Vazquez E; San Jose I; Giraldez F; Klein R; Represa J. 1995. Developing inner ear sensory neurons require TrkB and TrkC receptors for innervation of their peripheral targets. Development 121(10):3381-91PubMed: 7588071 MGI: J:29245
Minichiello L; Piehl F; Vazquez E; Schimmang T; Hokfelt T; Represa J; Klein R. 1995. Differential effects of combined trk receptor mutations on dorsal root ganglion and inner ear sensory neurons. Development 121(12):4067-75PubMed: 8575307 MGI: J:30344
White FA; Silos-Santiago I; Molliver DC; Nishimura M; Phillips H; Barbacid M; Snider WD. 1996. Synchronous onset of NGF and TrkA survival dependence in developing dorsal root ganglia. J Neurosci 16(15):4662-72PubMed: 8764654 MGI: J:34321
Fagan AM; Zhang H; Landis S; Smeyne RJ; Silos-Santiago I; Barbacid M. 1996. TrkA, but not TrkC, receptors are essential for survival of sympathetic neurons in vivo. J Neurosci 16(19):6208-18PubMed: 8815902 MGI: J:35563

Additional - Ntrk3^tm1Bbd related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Ntrk3^tm1Bbd
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, this mutant can be maintained by mating heterozygous siblings or by mating heterozygous mice with normal wildtype siblings. Homozygous mice die by 2-3 weeks of age.
- Additional Breeding and Husbandry Support
Citation
When using the trkC^TKcite the originating article(s) and include JAX stock #002481 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Ntrk3<tm1Bbd>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: trkCTK Mice homozygous for this Ntrk3tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ntrk3tm1Bbd

Allele Symbol: Ntrk3tm1Bbd

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ntrk3tm1Bbd related

Mammalian Phenotype Terms by Genotype

Genotype: Ntrk3tm1Bbd/Ntrk3tm1Bbdinvolves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

mammalian phenotype

mortality/aging

nervous system phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

References

Additional References

Additional - Ntrk3tm1Bbd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: trkC^TK
Mice homozygous for this Ntrk3^tm1 targeted mutation (commonly referred to as trkC) die by 2-3 weeks of age. Homozygous mutant mice lack proprioceptive and cochlear neurons and have a reduction in vestibular neurons.

Ntrk3^tm1Bbd

Allele Symbol: Ntrk3^tm1Bbd

Genotype: Ntrk3^tm1Bbd related

Genotype: Ntrk3^tm1Bbd/Ntrk3^tm1Bbd
involves: 129S2/SvPas * C57BL/6

Additional - Ntrk3^tm1Bbd related