IL-2Rgamma- (γc) mice are useful when studying the development and function of B-cells, T-cells, and NK-cells.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Mice homozygous for the Il2rgtm1Cgn targeted mutation are viable and fertile. Mutant mice have hypoplastic thymuses with a 10-fold reduction in the absolute number of lymphocytes. They have a limited number of mature splenic B and T cells, lack NK cells and Peyer's patches, and development of gut-associated intraepithelial lymphocytes is diminished. In vitro studies show defects in NK activity, IL4-directed Ig class switching of thymocytes, and thymocyte mitogenic responses.
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 6 of the interleukin 2 receptor, gamma chain (Il2rg) gene. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pIC-Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exon 4, intact floxed-neo cassette, or excision of both exon 4 and the neo cassette. Correctly targeted ES cells, with excision of exons 2-6 and the neo cassette, were injected into CB20 blastocysts and resulting chimeric mice were bred.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||cgamma-; gamma-; gammac-; gc-; Il2rg-; Il2rgtm1Rsky; IL-2Rgamma-|
|Gene Symbol and Name||Il2rg, interleukin 2 receptor, gamma chain|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Insertion of a neomycin resistance cassette flanked by lox P sites, followed by homologous recombination and cre-mediated deletion, resulted in the excision of exons 2 - 6 of the gene. These exons encode the extracellular and transmembrane domains of the protein.|
|Mutations Made By|| |
James DiSanto, University of Cologne
The Il2rgtm1Cgn targeted mutation is on the X chromosome. When maintaining a live colony, homozygous females may be bred to hemizygous males.
When using the IL2Rγnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #002479 in your Materials and Methods section.