B6;129S-Syt1^tm1Sud/J

Stock number: 002478
Research areas: Neurobiology Research

Detailed description

Mice homozygous for the Syt1^tm1Sud targeted mutation die 1-2 days after birth. Homozygous mutant mice show an impaired synchronous, fast Ca²⁺-dependent neurotransmitter release.

Allele

Symbol: Syt1^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:1857252
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Syt1
Marker name: synaptotagmin I
Chromosome: 10
Strain of origin: 129S4/SvJae
Molecular note: A 5' portion of the exon encoding amino acids 270 through 308 and the preceding intron were replaced with a neomycin selection cassette. In addition to the coding sequence, the intron acceptor site was deleted. Lysates derived from the brains of homozygous mice were shown to contain less than 5% of the amount of a truncated form of the encoded protein compared to controls by immunoblotting.